Overview

Product Description	Rabbit Polyclonal antibody recognizes CKAP5
Tested Reactivity	Hu, Ms, Rat
Tested Application	ICC/IF, IP, WB
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Target Name	CKAP5
Antigen Species	Human
Immunogen	Recombinant protein of Human CKAP5.
Conjugation	Un-conjugated
Alternate Names	Colonic and hepatic tumor overexpressed gene protein; TOGp; MSPS; Ch-TOG; Cytoskeleton-associated protein 5; CHTOG; TOG; ch-TOG

Application Instructions

Application Suggestion	Tested Application Dilution ICC/IF 1:50 - 1:200 IP Assay-dependent WB 1:500 - 1:2000
Application Note	* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control	HT-29

Properties

Form	Liquid
Purification	Affinity purification with immunogen.
Buffer	PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol.
Preservative	0.02% Sodium azide
Stabilizer	50% Glycerol
Storage Instruction	For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note	For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links	GeneID: 75786 Mouse CKAP5 GeneID: 9793 Human CKAP5 Swiss-port # A2AGT5 Mouse Cytoskeleton-associated protein 5 Swiss-port # Q14008 Human Cytoskeleton-associated protein 5
Gene Symbol	CKAP5
Gene Full Name	cytoskeleton associated protein 5
Background	This gene encodes a cytoskeleton-associated protein which belongs to the TOG/XMAP215 family. The N-terminal half of this protein contains a microtubule-binding domain and the C-terminal half contains a KXGS motif for binding tubulin dimers. This protein has two distinct roles in spindle formation; it protects kinetochore microtubules from depolymerization and plays an essential role in centrosomal microtubule assembly. This protein may be necessary for the proper interaction of microtubules with the cell cortex for directional cell movement. It also plays a role in translation of the myelin basic protein (MBP) mRNA by interacting with heterogeneous nuclear ribonucleoprotein (hnRNP) A2, which associates with MBP. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Aug 2011]
Function	Binds to the plus end of microtubules and regulates microtubule dynamics and microtubule organization. Promotes cytoplasmic microtubule nucleation and elongation. Plays a major role in organizing spindle poles. [UniProt]
Calculated MW	225 kDa

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