ARG58557
anti-CK2 beta antibody
anti-CK2 beta antibody for Western blot and Human,Mouse,Pig
Overview
| Product Description | Goat Polyclonal antibody recognizes CK2 beta |
|---|---|
| Tested Reactivity | Hu, Ms, Pig |
| Predict Reactivity | Cow, Rat, Dog |
| Tested Application | WB |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | CK2 beta |
| Antigen Species | Human |
| Immunogen | Synthetic peptide from the internal region of Human CK2 beta. (NP_001311.3; NP_001269314.1). (C-PEYRPKRPANQFVPR) |
| Conjugation | Un-conjugated |
| Alternate Names | G5A; Phosvitin; CSK2B; Casein kinase II subunit beta; Protein G5a; CK II beta; CK2N; CK2B |
Application Instructions
| Application Suggestion |
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| Application Note | WB: Recommend incubate at RT for 1h. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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| Observed Size | ~ 26 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Ammonium sulphate precipitation followed by affinity purification with immunogen. |
| Buffer | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 0.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | CSNK2B |
| Gene Full Name | casein kinase 2, beta polypeptide |
| Background | This gene encodes the beta subunit of casein kinase II, a ubiquitous protein kinase which regulates metabolic pathways, signal transduction, transcription, translation, and replication. The enzyme is composed of three subunits, alpha, alpha prime and beta, which form a tetrameric holoenzyme. The alpha and alpha prime subunits are catalytic, while the beta subunit serves regulatory functions. The enzyme localizes to the endoplasmic reticulum and the Golgi apparatus. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2013] |
| Function | Participates in Wnt signaling (By similarity). Plays a complex role in regulating the basal catalytic activity of the alpha subunit. [UniProt] |
| Calculated MW | 25 kDa |
| PTM | Phosphorylated by alpha subunit. [UniProt] |
Images (2) Click the Picture to Zoom In
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ARG58557 anti-CK2 beta antibody WB image
Western blot: 35 µg of NIH/3T3, HeLa, K562, MCF7 and HepG2 cell lysates (in RIPA buffer) stained with ARG58557 anti-CK2 beta antibody at 0.3 µg/ml dilution. Primary incubation was 1 hour. Detected by chemiluminescence.
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ARG58557 anti-CK2 beta antibody WB image
Western blot: 35 µg of Pig testis lysate (in RIPA buffer) stained with ARG58557 anti-CK2 beta antibody at 0.3 µg/ml dilution. Primary incubation was 1 hour. Detected by chemiluminescence.
