ARG64445
anti-CK1 epsilon antibody
anti-CK1 epsilon antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
Signaling Transduction antibody
Overview
| Product Description | Goat Polyclonal antibody recognizes CK1 epsilon |
|---|---|
| Tested Reactivity | Hu |
| Predict Reactivity | Ms, Rat, Dog |
| Tested Application | IHC-P, WB |
| Specificity | Both reported variants represent identical protein (NP_001885.1 and NP_689407.1). |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | CK1 epsilon |
| Antigen Species | Human |
| Immunogen | C-PASQTSVPFDHLGK |
| Conjugation | Un-conjugated |
| Alternate Names | tau; CK1epsilon; Casein kinase I isoform epsilon; AI426939; CKIe; CKI-epsilon; AW457082; AI551861; EC 2.7.11.1; KC1epsilon |
Application Instructions
| Application Suggestion |
|
||||||
|---|---|---|---|---|---|---|---|
| Application Note | WB: Recommend incubate at RT for 1h. IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Buffer | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 0.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Background | The protein encoded by this gene is a serine/threonine protein kinase and a member of the casein kinase I protein family, whose members have been implicated in the control of cytoplasmic and nuclear processes, including DNA replication and repair. The encoded protein is found in the cytoplasm as a monomer and can phosphorylate a variety of proteins, including itself. This protein has been shown to phosphorylate period, a circadian rhythm protein. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008] |
| Research Area | Signaling Transduction antibody |
| Calculated MW | 47 kDa |
Images (3) Click the Picture to Zoom In
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ARG64445 anti-CK1 epsilon antibody WB image
Western blot: 1) Mock transfection and 2) CSNK1E expressing plasmid transfected HEK293 cell lysate stained with ARG64445 anti-CK1 epsilon antibody.
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ARG64445 anti-CK1 epsilon antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human tonsil tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG64445 anti-CK1 epsilon antibody at 5 µg/ml dilution followed by AP-staining.
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ARG64445 anti-CK1 epsilon antibody WB image
Western blot: 35 µg of HEK293 cell lysate (in RIPA buffer) stained with ARG64445 anti-CK1 epsilon antibody at 1 µg/ml dilution and incubated at RT for 1 hour.
