ARG10682

anti-CK1 alpha antibody

anti-CK1 alpha antibody for ICC/IF,IHC-Frozen sections,Western blot and Human,Mouse,Rat,Cow,Horse,Pig

Overview

Product Description Chicken Polyclonal antibody recognizes CK1 alpha
Tested Reactivity Hu, Ms, Rat, Cow, Hrs, Pig
Predict Reactivity Chk
Tested Application ICC/IF, IHC-Fr, WB
Host Chicken
Clonality Polyclonal
Isotype IgY
Target Name CK1 alpha
Immunogen Recombinant full length CSNK1A1 protein (shortest isoform).
Conjugation Un-conjugated
Alternate Names CK1a; PRO2975; HEL-S-77p; CKIa; HLCDGP1; CK1; CKI-alpha; EC 2.7.11.1; Casein kinase I isoform alpha

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IF1:500 - 1:1000
IHC-FrAssay-dependent
WB1:10000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Buffer PBS and 0.02% Sodium azide.
Preservative 0.02% Sodium azide
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 113927 Rat CSNK1A1

GeneID: 1452 Human CSNK1A1

GeneID: 93687 Mouse CSNK1A1

Gene Symbol CSNK1A1
Gene Full Name casein kinase 1, alpha 1
Function Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. It can phosphorylate a large number of proteins. Participates in Wnt signaling. Phosphorylates CTNNB1 at 'Ser-45'. May phosphorylate PER1 and PER2. May play a role in segregating chromosomes during mitosis. [UniProt]
Calculated MW 39 kDa

Images (2) Click the Picture to Zoom In

  • ARG10682 anti-CK1 alpha antibody ICC/IF image

    Immunocytochemistry: HeLa cells stained with ARG10682 anti-CK1 alpha antibody (red) and co-stained with panspecific Mouse monoclonal antibody to nuclear pore complexes (green). Casein kinase 1 alpha has a particulate distribution both in the nucleus and the cytoplasm. Blue is the Hoechst DNA stain.

  • ARG10682 anti-CK1 alpha antibody WB image

    Western blot: Whole Rat spinal cord homogenate stained with ARG10682 anti-CK1 alpha antibody at 1:10000 dilution. A prominent band running with an apparent SDS-PAGE molecular weight of ~38 kDa and a less prominent band at ~34 kDa corresponds to other CK1 alpha isotypes.