ARG64007
anti-CILP antibody
anti-CILP antibody for Western blot and Rat
Immune System antibody; Signaling Transduction antibody
Overview
| Product Description | Goat Polyclonal antibody recognizes CILP |
|---|---|
| Tested Reactivity | Rat |
| Predict Reactivity | Hu, Ms, Dog |
| Tested Application | WB |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | CILP |
| Antigen Species | Human |
| Immunogen | C-NYRRTDHEDPRVK |
| Conjugation | Un-conjugated |
| Alternate Names | Cartilage intermediate layer protein 1; Cartilage intermediate-layer protein; CILP-1; HsT18872 |
Application Instructions
| Application Suggestion |
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|---|---|---|---|---|---|
| Application Note | WB: Recommend incubate at RT for 1h. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Buffer | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 0.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Background | Major alterations in the composition of the cartilage extracellular matrix occur in joint disease, such as osteoarthrosis. This gene encodes the cartilage intermediate layer protein (CILP), which increases in early osteoarthrosis cartilage. The encoded protein was thought to encode a protein precursor for two different proteins; an N-terminal CILP and a C-terminal homolog of NTPPHase, however, later studies identified no nucleotide pyrophosphatase phosphodiesterase (NPP) activity. The full-length and the N-terminal domain of this protein was shown to function as an IGF-1 antagonist. An allelic variant of this gene has been associated with lumbar disc disease. [provided by RefSeq, Sep 2010] |
|---|---|
| Research Area | Immune System antibody; Signaling Transduction antibody |
| Calculated MW | 133 kDa |
| PTM | Cleaved into 2 chains possibly by a furin-like protease upon or preceding secretion. |
Images (1) Click the Picture to Zoom In
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ARG64007 anti-CILP antibody WB image
Western Blot: rat spinal cord lysate (35 µg protein in RIPA buffer) stained with ARG64007 anti-CILP antibody at 0.03 µg/ml dilution.
