ARG58425
anti-CHRNE antibody
anti-CHRNE antibody for Flow cytometry,Western blot and Human,Mouse,Rat
Overview
| Product Description | Rabbit Polyclonal antibody recognizes CHRNE |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | FACS, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | CHRNE |
| Antigen Species | Human |
| Immunogen | KLH-conjugated synthetic peptide corresponding to aa. 409-443 of Human CHRNE. |
| Conjugation | Un-conjugated |
| Alternate Names | FCCMS; CMS1E; CMS1D; SCCMS; CMS2A; Acetylcholine receptor subunit epsilon; CMS4B; CMS4C; ACHRE; CMS4A |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| Positive Control | Human skeletal muscle |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein A and immunogen peptide. |
| Buffer | PBS and 0.09% (W/V) Sodium azide. |
| Preservative | 0.09% (W/V) Sodium azide |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | CHRNE |
| Gene Full Name | cholinergic receptor, nicotinic, epsilon (muscle) |
| Background | Acetylcholine receptors at mature mammalian neuromuscular junctions are pentameric protein complexes composed of four subunits in the ratio of two alpha subunits to one beta, one epsilon, and one delta subunit. The acetylcholine receptor changes subunit composition shortly after birth when the epsilon subunit replaces the gamma subunit seen in embryonic receptors. Mutations in the epsilon subunit are associated with congenital myasthenic syndrome. [provided by RefSeq, Sep 2009] |
| Function | After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane. [UniProt] |
| Calculated MW | 55 kDa |
Images (2) Click the Picture to Zoom In
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ARG58425 anti-CHRNE antibody WB image
Western blot: 20 µg of Human skeletal muscle lysate stained with ARG58425 anti-CHRNE antibody at 1:2000 dilution.
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ARG58425 anti-CHRNE antibody FACS image
Flow Cytometry: HepG2 cells were fixed with 2% paraformaldehyde (10 min). The cells were then incubated in 2% BSA to block non-specific protein-protein interactions and stained with ARG58425 anti-CHRNE antibody (right histogram) at 1:25 dilution for 60 min at 37°C, followed by incubation with DyLight® 488 labelled secondary antibody. Isotype control antibody (left histogram) was Rabbit IgG1 (1 µg/10^6 cells) used under the same conditions. Acquisition of >10000 events was performed.
