ARG64681
anti-CHRNA4 antibody
anti-CHRNA4 antibody for Western blot and Human,Rat
Cancer antibody; Metabolism antibody; Neuroscience antibody
Overview
| Product Description | Goat Polyclonal antibody recognizes CHRNA4 |
|---|---|
| Tested Reactivity | Hu, Rat |
| Predict Reactivity | Ms, Cow, Dog |
| Tested Application | WB |
| Specificity | This antibody is expected NOT to cross-react with the similar alpha 2 subunit. |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | CHRNA4 |
| Antigen Species | Human |
| Immunogen | C-HVETRAHAEERLLKK |
| Conjugation | Un-conjugated |
| Alternate Names | Neuronal acetylcholine receptor subunit alpha-4; NACRA4; NACHRA4; EBN; BFNC; EBN1; NACHR |
Application Instructions
| Application Suggestion |
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|---|---|---|---|---|---|
| Application Note | WB: Recommend incubate at RT for 1h. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Buffer | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 0.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P09483 Rat Neuronal acetylcholine receptor subunit alpha-4 Swiss-port # P43681 Human Neuronal acetylcholine receptor subunit alpha-4 |
|---|---|
| Background | This gene encodes a nicotinic acetylcholine receptor, which belongs to a superfamily of ligand-gated ion channels that play a role in fast signal transmission at synapses. These pentameric receptors can bind acetylcholine, which causes an extensive change in conformation that leads to the opening of an ion-conducting channel across the plasma membrane. This protein is an integral membrane receptor subunit that can interact with either nAChR beta-2 or nAChR beta-4 to form a functional receptor. Mutations in this gene cause nocturnal frontal lobe epilepsy type 1. Polymorphisms in this gene that provide protection against nicotine addiction have been described. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2012] |
| Research Area | Cancer antibody; Metabolism antibody; Neuroscience antibody |
| Calculated MW | 70 kDa |
Images (1) Click the Picture to Zoom In
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ARG64681 anti-CHRNA4 antibody WB image
Western Blot: Rat Brain lysate (35 µg protein in RIPA buffer) stained with ARG64681 anti-CHRNA4 (aa29-43) antibody at 0.3 µg/ml dilution.
