ARG41846
anti-CHRM2 antibody
anti-CHRM2 antibody for Western blot and Mouse
Overview
| Product Description | Goat Polyclonal antibody recognizes CHRM2 |
|---|---|
| Tested Reactivity | Ms |
| Predict Reactivity | Hu, Cow, Dog, Pig |
| Tested Application | WB |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | CHRM2 |
| Antigen Species | Human |
| Immunogen | Synthetic peptide around the internal region of Human CHRM2. (C-QNGDEKQNIVARK) (NP_000730.1) |
| Conjugation | Un-conjugated |
| Alternate Names | Muscarinic acetylcholine receptor M2; HM2 |
Application Instructions
| Application Suggestion |
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| Application Note | WB: Recommend incubate at RT for 1h. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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| Observed Size | ~ 50 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Ammonium sulphate precipitation followed by affinity purification with immunogen. |
| Buffer | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 0.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # Q9ERZ4 Mouse Muscarinic acetylcholine receptor M2 |
|---|---|
| Gene Symbol | CHRM2 |
| Gene Full Name | cholinergic receptor, muscarinic 2 |
| Background | The muscarinic cholinergic receptors belong to a larger family of G protein-coupled receptors. The functional diversity of these receptors is defined by the binding of acetylcholine to these receptors and includes cellular responses such as adenylate cyclase inhibition, phosphoinositide degeneration, and potassium channel mediation. Muscarinic receptors influence many effects of acetylcholine in the central and peripheral nervous system. The muscarinic cholinergic receptor 2 is involved in mediation of bradycardia and a decrease in cardiac contractility. Multiple alternatively spliced transcript variants have been described for this gene. [provided by RefSeq, Jul 2008] |
| Function | The muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Primary transducing effect is adenylate cyclase inhibition. Signaling promotes phospholipase C activity, leading to the release of inositol trisphosphate (IP3); this then triggers calcium ion release into the cytosol. [UniProt] |
| Cellular Localization | Cell membrane; Multi-pass membrane protein. Cell junction, synapse, postsynaptic cell membrane; Multi-pass membrane protein. Note=Phosphorylation in response to agonist binding promotes receptor internalization. [UniProt] |
| Calculated MW | 52 kDa |
| PTM | Phosphorylated in response to agonist treatment. [UniProt] |
Images (1) Click the Picture to Zoom In
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ARG41846 anti-CHRM2 antibody WB image
Western blot: 35 µg of Mouse brain lysate (in RIPA buffer) stained with ARG41846 anti-CHRM2 antibody at 2 µg/ml dilution and incubated at RT for 1 hour.
