ARG66466
anti-CHRAC17 antibody
anti-CHRAC17 antibody for ICC/IF,Western blot and Human
Overview
| Product Description | Rabbit Polyclonal antibody recognizes CHRAC17 |
|---|---|
| Tested Reactivity | Hu |
| Predict Reactivity | Ms, Rat |
| Tested Application | ICC/IF, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | CHRAC17 |
| Antigen Species | Human |
| Immunogen | Recombinant full length protein of Human CHRAC17. |
| Conjugation | Un-conjugated |
| Alternate Names | DNA polymerase II subunit 3; CHRAC-17; HuCHRAC17; Chromatin accessibility complex 17 kDa protein; YBL1; AsTP; DNA polymerase epsilon subunit p17; Arsenic-transactivated protein; p17; CHARAC17; CHRAC17; EC 2.7.7.7; DNA polymerase epsilon subunit 3 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| Observed Size | ~ 17 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification with immunogen. |
| Buffer | 0.42% Potassium phosphate (pH 7.3), 0.87% NaCl, 0.01% Sodium azide and 30% Glycerol. |
| Preservative | 0.01% Sodium azide |
| Stabilizer | 30% Glycerol |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | POLE3 |
| Gene Full Name | polymerase (DNA directed), epsilon 3, accessory subunit |
| Background | POLE3 is a histone-fold protein that interacts with other histone-fold proteins to bind DNA in a sequence-independent manner. These histone-fold protein dimers combine within larger enzymatic complexes for DNA transcription, replication, and packaging.[supplied by OMIM, Apr 2004] |
| Function | Forms a complex with DNA polymerase epsilon subunit CHRAC1 and binds naked DNA, which is then incorporated into chromatin, aided by the nucleosome-remodeling activity of ISWI/SNF2H and ACF1. [UniProt] |
| Cellular Localization | Nucleus. [UniProt] |
| Calculated MW | 17 kDa |
Images (2) Click the Picture to Zoom In
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ARG66466 anti-CHRAC17 antibody ICC/IF image
Immunofluorescence: Formalin-fixed U2OS cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were stained with ARG66466 anti-CHRAC17 antibody (red) in 3% BSA-PBS and incubated overnight at 4°C in a hidified chamber.
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ARG66466 anti-CHRAC17 antibody WB image
Western blot: MCF7 whole cell lysate stained with ARG66466 anti-CHRAC17 antibody.
