ARG40721

anti-CHMP5 antibody

anti-CHMP5 antibody for Western blot and Human

Overview

Product Description Rabbit Polyclonal antibody recognizes CHMP5
Tested Reactivity Hu
Tested Application WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name CHMP5
Antigen Species Human
Immunogen Recombinant protein corresponding to aa. 1-219 of Human CHMP5.
Conjugation Un-conjugated
Alternate Names hVps60; Charged multivesicular body protein 5; SNF7 domain-containing protein 2; HSPC177; CGI-34; Vps60; PNAS-2; Chromatin-modifying protein 5; Vacuolar protein sorting-associated protein 60; SNF7DC2; C9orf83

Application Instructions

Application Suggestion
Tested Application Dilution
WB2 µg/ml
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Observed Size 25 kDa

Properties

Form Liquid
Purification Caprylic acid ammonium sulfate precipitation.
Buffer 0.01M PBS (pH 7.4), 0.03% Proclin 300 and 50% Glycerol.
Preservative 0.03% Proclin 300
Stabilizer 50% Glycerol
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 51510 Human CHMP5

Swiss-port # Q9NZZ3 Human Charged multivesicular body protein 5

Gene Symbol CHMP5
Gene Full Name charged multivesicular body protein 5
Background CHMP5 belongs to the chromatin-modifying protein/charged multivesicular body protein (CHMP) family. These proteins are components of ESCRT-III (endosomal sorting complex required for transport III), a complex involved in degradation of surface receptor proteins and formation of endocytic multivesicular bodies (MVBs). Some CHMPs have both nuclear and cytoplasmic/vesicular distributions, and one such CHMP, CHMP1A (MIM 164010), is required for both MVB formation and regulation of cell cycle progression (Tsang et al., 2006 [PubMed 16730941]).[supplied by OMIM, Mar 2008]
Function Probable peripherally associated component of the endosomal sorting required for transport complex III (ESCRT-III) which is involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs. MVBs contain intraluminal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids. The MVB pathway appears to require the sequential function of ESCRT-O, -I,-II and -III complexes. ESCRT-III proteins mostly dissociate from the invaginating membrane before the ILV is released. The ESCRT machinery also functions in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis and the budding of enveloped viruses (HIV-1 and other lentiviruses). ESCRT-III proteins are believed to mediate the necessary vesicle extrusion and/or membrane fission activities, possibly in conjunction with the AAA ATPase VPS4. Involved in HIV-1 p6- and p9-dependent virus release. [UniProt]
Cellular Localization Cytoplasm, cytosol. Endosome membrane; Peripheral membrane protein. Note=Localizes to the midbody of dividing cells. Localized in two distinct rings on either side of the Fleming body. [UniProt]
Calculated MW 25 kDa
PTM ISGylated. Isgylation inhibits its interaction with VTA1. [UniProt]

Images (1) Click the Picture to Zoom In

  • ARG40721 anti-CHMP5 antibody WB image

    Western blot: 293T and K562 whole cell lysates stained with ARG40721 anti-CHMP5 antibody at 2 µg/ml dilution.