Overview

Product Description	Rabbit Polyclonal antibody recognizes CHD4
Tested Reactivity	Hu
Tested Application	FACS, ICC/IF, WB
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Target Name	CHD4
Antigen Species	Human
Immunogen	Synthetic peptide of Human CHD4.
Conjugation	Un-conjugated
Alternate Names	Chromodomain-helicase-DNA-binding protein 4; Mi-2b; EC 3.6.4.12; Mi-2 autoantigen 218 kDa protein; CHD-4; Mi2-beta; ATP-dependent helicase CHD4; Mi2-BETA

Application Instructions

Application Suggestion	Tested Application Dilution FACS 1:50 ICC/IF 1:50 - 1:200 WB 1:1000 - 1:5000
Application Note	* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control	293T

Properties

Form	Liquid
Purification	Affinity purified.
Buffer	PBS (pH 7.4), 150 mM NaCl, 0.02% Sodium azide and 50% Glycerol.
Preservative	0.02% Sodium azide
Stabilizer	50% Glycerol
Storage Instruction	For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note	For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links	GeneID: 1108 Human CHD4 Swiss-port # Q14839 Human Chromodomain-helicase-DNA-binding protein 4
Gene Symbol	CHD4
Gene Full Name	chromodomain helicase DNA binding protein 4
Background	The product of this gene belongs to the SNF2/RAD54 helicase family. It represents the main component of the nucleosome remodeling and deacetylase complex and plays an important role in epigenetic transcriptional repression. Patients with dermatomyositis develop antibodies against this protein. Somatic mutations in this gene are associated with serous endometrial tumors. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2014]
Function	Component of the histone deacetylase NuRD complex which participates in the remodeling of chromatin by deacetylating histones. [UniProt]
Cellular Localization	Nucleus. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Note=Associates with centrosomes in interphase. [UniProt]
Calculated MW	218 kDa

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