ARG59833
anti-CHCHD4 / MIA40 antibody
anti-CHCHD4 / MIA40 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
Overview
| Product Description | Rabbit Polyclonal antibody recognizes CHCHD4 / MIA40 |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | CHCHD4 / MIA40 |
| Antigen Species | Human |
| Immunogen | Recombinant fusion protein corresponding to aa. 1-142 of Human CHCHD4 (NP_001091972.1). |
| Conjugation | Un-conjugated |
| Alternate Names | Coiled-coil-helix-coiled-coil-helix domain-containing protein 4; TIMM40; MIA40; Mitochondrial intermembrane space import and assembly protein 40 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| Positive Control | Mouse pancreas and 293T | ||||||
| Observed Size | 20 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purified. |
| Buffer | PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 50% Glycerol |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | CHCHD4 |
| Gene Full Name | coiled-coil-helix-coiled-coil-helix domain containing 4 |
| Background | CHCHD4, a component of human mitochondria, belongs to a protein family whose members share 6 highly conserved cysteine residues constituting a -CXC-CX(9)C-CX(9)C- motif in the C terminus (Hofmann et al., 2005 [PubMed 16185709]).[supplied by OMIM, Mar 2008] |
| Function | Functions as chaperone and catalyzes the formation of disulfide bonds in substrate proteins, such as COX17. Required for the import and folding of small cysteine-containing proteins (small Tim) in the mitochondrial intermembrane space (IMS). Precursor proteins to be imported into the IMS are translocated in their reduced form into the mitochondria. The oxidized form of CHCHD4/MIA40 forms a transient intermolecular disulfide bridge with the reduced precursor protein, resulting in oxidation of the precursor protein that now contains an intramolecular disulfide bond and is able to undergo folding in the IMS. Reduced CHCHD4/MIA40 is then reoxidized by GFER/ERV1 via a disulfide relay system. [UniProt] |
| Cellular Localization | Mitochondrion intermembrane space. Mitochondrion. [UniProt] |
| Calculated MW | 16 kDa |
| PTM | Forms intrachain disulfide bridges, but exists in different redox states. [UniProt] |
Images (1) Click the Picture to Zoom In
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ARG59833 anti-CHCHD4 / MIA40 antibody WB image
Western blot: 25 µg of Mouse pancreas and 293T cell lysates stained with ARG59833 anti-CHCHD4 / MIA40 antibody at 1:3000 dilution.
