ARG52241
anti-CGRP antibody [4901]
anti-CGRP antibody [4901] for Flow cytometry,ICC/IF,IHC-Frozen sections and Human,Mouse,Rat
Cancer antibody; Cell Biology and Cellular Response antibody; Neuroscience antibody; Signaling Transduction antibody
Overview
| Product Description | Mouse Monoclonal antibody [4901] recognizes CGRP |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Predict Reactivity | Mamm |
| Tested Application | FACS, ICC/IF, IHC-Fr |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | 4901 |
| Isotype | IgG2a |
| Target Name | CGRP |
| Antigen Species | Rat |
| Immunogen | Purified rat alpha-CGRP |
| Conjugation | Un-conjugated |
| Alternate Names | CALC1; CGRP-I; Calcitonin gene-related peptide 1; CGRP1; Calcitonin gene-related peptide I; Alpha-type CGRP; CGRP |
Application Instructions
| Application Suggestion |
|
||||||||
|---|---|---|---|---|---|---|---|---|---|
| Application Note | Specific for Calcitonin Gene Related Peptide. Also recognizes Rat beta CGRP and human alpha-CGRP (10-fold less sensitivity compared to Rat alpha-CGRP). Suggested positive controls are dorsal root ganglia, pancreas and gut. *This antibody is an excellent tool for immunohistochemistry and for multiple labeling immunofluorescence of peripheral tissue like stomach, intestine, pancreas, sensory ganglia, and all the regions of the body that are innervated by primary afferent neurons as well as central nervous tissue. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Protein G purified |
| Buffer | 10 mM HEPES (pH 7.5), 150 mM NaCl, 0.1 mg/ml BSA and 50% Glycerol |
| Stabilizer | 0.1 mg/ml BSA, 50% Glycerol |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | CALCA |
| Gene Full Name | Calcitonin gene-related peptide 1 |
| Background | This gene encodes the peptide hormones calcitonin, calcitonin gene-related peptide and katacalcin by tissue-specific alternative RNA splicing of the gene transcripts and cleavage of inactive precursor proteins. Calcitonin is involved in calcium regulation and acts to regulate phosphorus metabolism. Calcitonin gene-related peptide functions as a vasodilator and as an antimicrobial peptide while katacalcin is a calcium-lowering peptide. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2014] |
| Function | CGRP induces vasodilation. It dilates a variety of vessels including the coronary, cerebral and systemic vasculature. Its abundance in the CNS also points toward a neurotransmitter or neuromodulator role. It also elevates platelet cAMP. [UniProt] |
| Research Area | Cancer antibody; Cell Biology and Cellular Response antibody; Neuroscience antibody; Signaling Transduction antibody |
| Calculated MW | 14 kDa |
Clone References
Defining the nociceptor transcriptome.
ICC/IF / Mouse
Neuroprotective effect of erythropoietin against pressure ulcer in a mouse model of small fiber neuropathy.
IHC-Fr / Mouse
A paracrine network regulates the cross-talk between human lung stem cells and the stroma.
FACS / Human
Oxytocin is expressed by both intrinsic sensory and secretomotor neurons in the enteric nervous system of guinea pig.
IHC / Guinea pig
Differential distribution of calcitonin gene-related peptide and its receptor components in the human trigeminal ganglion.
IHC-Fr / Human, Rat
Neurochemical diversity of afferent neurons that transduce sensory signals from dog ventricular myocardium.
IHC-Fr / Dog
