ARG64156
anti-CGI58 / ABHD5 antibody
anti-CGI58 / ABHD5 antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse
Cancer antibody; Cell Biology and Cellular Response antibody; Metabolism antibody; Signaling Transduction antibody
Overview
| Product Description | Goat Polyclonal antibody recognizes CGI58 / ABHD5 |
|---|---|
| Tested Reactivity | Hu, Ms |
| Predict Reactivity | Dog, Rat |
| Tested Application | ICC/IF, IHC-P, WB |
| Specificity | This antibody is not expected to cross-react with ABHD4. |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | CGI58 / ABHD5 |
| Antigen Species | Human |
| Immunogen | C-FPERPDLADQDR |
| Conjugation | Un-conjugated |
| Alternate Names | Lipid droplet-binding protein CGI-58; CGI58; 1-acylglycerol-3-phosphate O-acyltransferase ABHD5; NCIE2; EC 2.3.1.51; CDS; IECN2; Abhydrolase domain-containing protein 5 |
Application Instructions
| Application Suggestion |
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|---|---|---|---|---|---|---|---|---|---|
| Application Note | WB: Recommend incubate at RT for 1h. IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Buffer | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 0.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # Q8WTS1 Human 1-acylglycerol-3-phosphate O-acyltransferase ABHD5 Swiss-port # Q9DBL9 Mouse 1-acylglycerol-3-phosphate O-acyltransferase ABHD5 |
|---|---|
| Background | The protein encoded by this gene belongs to a large family of proteins defined by an alpha/beta hydrolase fold, and contains three sequence motifs that correspond to a catalytic triad found in the esterase/lipase/thioesterase subfamily. It differs from other members of this subfamily in that its putative catalytic triad contains an asparagine instead of the serine residue. Mutations in this gene have been associated with Chanarin-Dorfman syndrome, a triglyceride storage disease with impaired long-chain fatty acid oxidation. [provided by RefSeq, Jul 2008] |
| Research Area | Cancer antibody; Cell Biology and Cellular Response antibody; Metabolism antibody; Signaling Transduction antibody |
| Calculated MW | 39 kDa |
Images (4) Click the Picture to Zoom In
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ARG64156 anti-CGI58 / ABHD5 antibody WB image
Western Blot: NIH3T3 lysate (35 µg protein in RIPA buffer) stained with ARG64156 anti-CGI58 / ABHD5 antibody at 0.2 µg/ml dilution.
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ARG64156 anti-CGI58 / ABHD5 antibody ICC/IF image
Immunofluorescence: Paraformaldehyde fixed U2OS cells permeabilized with 0.15% Triton. Cells were stained with ARG64156 anti-CGI58 / ABHD5 antibody (green) at 10 µg/ml dilution for 1 hour. DAPI (blue) for nuclear staining. Negative control: Unimmunized goat IgG (green) at 10 µg/ml dilution.
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ARG64156 anti-CGI58 / ABHD5 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human pancreas tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG64156 anti-CGI58 / ABHD5 antibody at 3.75 µg/ml dilution followed by AP-staining.
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ARG64156 anti-CGI58 / ABHD5 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human kidney tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG64156 anti-CGI58 / ABHD5 antibody at 3.75 µg/ml dilution followed by AP-staining.
