ARG62460
anti-CFTR antibody [MM13-4]
anti-CFTR antibody [MM13-4] for Flow cytometry,ICC/IF,Immunoprecipitation,Western blot and Human
Cell Biology and Cellular Response antibody; Metabolism antibody
Overview
| Product Description | Mouse Monoclonal antibody [MM13-4] recognizes CFTR |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | FACS, ICC/IF, IP, WB |
| Specificity | This antibody recognizes CFTR, Mr 170kDa. Two additional proteins were detected, Mr 52 and 38 kDa, in CFTR-transfected BHK cell lysates. |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | MM13-4 |
| Isotype | IgG1 |
| Target Name | CFTR |
| Antigen Species | Human |
| Immunogen | Synthetic peptide (RKGYRQRLELSD) corresponding to residues 25-36 of human cystic fibrosis transmembrane conductance regulator (CFTR) |
| Conjugation | Un-conjugated |
| Alternate Names | ATP-binding cassette sub-family C member 7; TNR-CFTR; ABC35; CFTR/MRP; CF; EC 3.6.3.49; dJ760C5.1; Channel conductance-controlling ATPase; cAMP-dependent chloride channel; ABCC7; Cystic fibrosis transmembrane conductance regulator; CFTR; MRP7 |
Application Instructions
| Application Note | FACS: 2 µg for 1 million cells WB: 10 µg/ml * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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Properties
| Form | Liquid |
|---|---|
| Purification | Protein G purified |
| Buffer | PBS (pH 7.6), 0.25M NaCl and 0.1% Sodium azide |
| Preservative | 0.1% Sodium azide |
| Concentration | 0.2 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P13569 Human Cystic fibrosis transmembrane conductance regulator |
|---|---|
| Gene Symbol | CFTR |
| Gene Full Name | cystic fibrosis transmembrane conductance regulator (ATP-binding cassette sub-family C, member 7) |
| Background | This gene encodes a member of the ATP-binding cassette (ABC) transporter superfamily. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MRP subfamily that is involved in multi-drug resistance. The encoded protein functions as a chloride channel and controls the regulation of other transport pathways. Mutations in this gene are associated with the autosomal recessive disorders cystic fibrosis and congenital bilateral aplasia of the vas deferens. Alternatively spliced transcript variants have been described, many of which result from mutations in this gene. [provided by RefSeq, Jul 2008] |
| Function | Involved in the transport of chloride ions. May regulate bicarbonate secretion and salvage in epithelial cells by regulating the SLC4A7 transporter. Can inhibit the chloride channel activity of ANO1. Plays a role in the chloride and bicarbonate homeostasis during sperm epididymal maturation and capacitation. [UniProt] |
| Cellular Localization | Early endosome membrane |
| Research Area | Cell Biology and Cellular Response antibody; Metabolism antibody |
| Calculated MW | 168 kDa |
| PTM | N-glycosylated. Phosphorylated; cAMP treatment promotes phosphorylation and activates the channel (PubMed:12588899, PubMed:17036051, PubMed:8910473). Dephosphorylation decreases the ATPase activity (in vitro) (PubMed:8910473). Phosphorylation at PKA sites activates the channel (PubMed:10792060, PubMed:12519745, PubMed:12588899, PubMed:25330774). Phosphorylation at PKC sites enhances the response to phosphorylation by PKA (PubMed:12588899). Phosphorylated by AMPK; this inhibits channel activity (PubMed:12519745). Ubiquitinated, leading to its degradation in the lysosome (PubMed:19398555). Deubiquitination by USP10 in early endosomes enhances its endocytic recycling to the cell membrane (PubMed:19398555). Ubiquitinated by RNF185 during ER stress (PubMed:24019521). |
