ARG64484
anti-CES1 antibody
anti-CES1 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
Cell Biology and Cellular Response antibody; Metabolism antibody; Signaling Transduction antibody
Overview
| Product Description | Goat Polyclonal antibody recognizes CES1 |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | IHC-P, WB |
| Specificity | This antibody is expected to recognise all three reported isoforms (NP_001020366.1; NP_001020365.1; NP_001257.4). |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | CES1 |
| Antigen Species | Human |
| Immunogen | C-NTQAAQKLKDKE |
| Conjugation | Un-conjugated |
| Alternate Names | SES1; Cocaine carboxylesterase; REH; ACAT; Serine esterase 1; HMSE; Retinyl ester hydrolase; Methylumbelliferyl-acetate deacetylase 1; hCE-1; EC 3.1.1.56; CE-1; CEH; HMSE1; TGH; PCE-1; Acyl-coenzyme A:cholesterol acyltransferase; EC 3.1.1.1; Liver carboxylesterase 1; Brain carboxylesterase hBr1; Triacylglycerol hydrolase; CES2; Egasyn; Carboxylesterase 1; Monocyte/macrophage serine esterase |
Application Instructions
| Application Suggestion |
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|---|---|---|---|---|---|---|---|
| Application Note | IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). WB: Recommend incubate at RT for 1h. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Buffer | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 0.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Background | This gene encodes a member of the carboxylesterase large family. The family members are responsible for the hydrolysis or transesterification of various xenobiotics, such as cocaine and heroin, and endogenous substrates with ester, thioester, or amide bonds. They may participate in fatty acyl and cholesterol ester metabolism, and may play a role in the blood-brain barrier system. This enzyme is the major liver enzyme and functions in liver drug clearance. Mutations of this gene cause carboxylesterase 1 deficiency. Three transcript variants encoding three different isoforms have been found for this gene. [provided by RefSeq, Jun 2010] |
| Research Area | Cell Biology and Cellular Response antibody; Metabolism antibody; Signaling Transduction antibody |
| Calculated MW | 63 kDa |
| PTM | Contains sialic acid. Cleavage of the signal sequence can occur at 2 positions, either between Trp-17 and Gly-18 or between Gly-18 and His-19. |
Images (2) Click the Picture to Zoom In
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ARG64484 anti-CES1 antibody WB image
Western Blot: Human Liver lysate (35 µg protein in RIPA buffer) stained with ARG64484 anti-CES1 antibody at 0.03 µg/ml dilution.
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ARG64484 anti-CES1 antibody IHC-P image
Immunohistochemistry: paraffin embedded Human Lung. (Steamed antigen retrieval with citrate buffer pH 6) stained with ARG64484 anti-CES1 antibody at 2.5 µg/ml dilution followed by AP-staining.
