ARG63565
anti-CENTB1 / ACAP1 antibody
anti-CENTB1 / ACAP1 antibody for Western blot and Human
Signaling Transduction antibody
Overview
| Product Description | Goat Polyclonal antibody recognizes CENTB1 / ACAP1 |
|---|---|
| Tested Reactivity | Hu |
| Predict Reactivity | Ms, Rat, Cow, Dog |
| Tested Application | WB |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | CENTB1 / ACAP1 |
| Antigen Species | Human |
| Immunogen | C-EKLSRRSHDLHTL |
| Conjugation | Un-conjugated |
| Alternate Names | Arf-GAP with coiled-coil, ANK repeat and PH domain-containing protein 1; Centaurin-beta-1; CENTB1; Cnt-b1 |
Application Instructions
| Application Suggestion |
|
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|---|---|---|---|---|---|
| Application Note | WB: Recommend incubate at RT for 1h. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Buffer | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 0.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # Q15027 Human Arf-GAP with coiled-coil, ANK repeat and PH domain-containing protein 1 |
|---|---|
| Gene Symbol | ACAP1 |
| Gene Full Name | ArfGAP with coiled-coil, ankyrin repeat and PH domains 1 |
| Function | GTPase-activating protein (GAP) for ADP ribosylation factor 6 (ARF6) required for clathrin-dependent export of proteins from recycling endosomes to trans-Golgi network and cell surface. Required for regulated export of ITGB1 from recycling endosomes to the cell surface and ITGB1-dependent cell migration. [UniProt] |
| Research Area | Signaling Transduction antibody |
| Calculated MW | 82 kDa |
| PTM | Phosphorylation at Ser-554 by PKB is required for interaction with ITGB1, export of ITGB1 from recycling endosomes to the cell surface and ITGB1-dependent cell migration. |
Images (1) Click the Picture to Zoom In
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ARG63565 anti-CENTB1 / ACAP1 antibody WB image
Western Blot: Jurkat lysate (RIPA buffer, 35µg total protein per lane) stained with ARG63565 anti-CENTB1 / ACAP1 antibody diluted in 0.5µg/ml.
