ARG56185
anti-CELA3B / Elastase 3B antibody [CELA3B/1218]
anti-CELA3B / Elastase 3B antibody [CELA3B/1218] for ELISA,Western blot and Human
Overview
| Product Description | Mouse Monoclonal antibody [CELA3B/1218] recognizes CELA3B / Elastase 3B |
|---|---|
| Tested Reactivity | Hu |
| Species Does Not React With | Rat |
| Tested Application | ELISA, WB |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | CELA3B/1218 |
| Isotype | IgG, kappa |
| Target Name | CELA3B / Elastase 3B |
| Antigen Species | Human |
| Immunogen | A partial recombinant CELA3B protein (aa. 82-238). |
| Conjugation | Un-conjugated |
| Alternate Names | ELA3B; CBPP; EC 3.4.21.70; Elastase-3B; Chymotrypsin-like elastase family member 3B; Elastase IIIB; EL-1; Protease E; E1 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein G. |
| Buffer | PBS (pH 7.4), 0.05% Sodium azide and 0.1 mg/ml BSA. |
| Preservative | 0.05% Sodium azide |
| Stabilizer | 0.1 mg/ml BSA |
| Concentration | 0.2 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P08861 Human Chymotrypsin-like elastase family member 3B |
|---|---|
| Gene Symbol | CELA3B |
| Gene Full Name | chymotrypsin-like elastase family, member 3B |
| Background | Elastases form a subfamily of serine proteases that hydrolyze many proteins in addition to elastin. Humans have six elastase genes which encode the structurally similar proteins elastase 1, 2, 2A, 2B, 3A, and 3B. Unlike other elastases, elastase 3B has little elastolytic activity. Like most of the human elastases, elastase 3B is secreted from the pancreas as a zymogen and, like other serine proteases such as trypsin, chymotrypsin and kallikrein, it has a digestive function in the intestine. Elastase 3B preferentially cleaves proteins after alanine residues. Elastase 3B may also function in the intestinal transport and metabolism of cholesterol. Both elastase 3A and elastase 3B have been referred to as protease E and as elastase 1, and excretion of this protein in fecal material is frequently used as a measure of pancreatic function in clinical assays. [provided by RefSeq, May 2009] |
| Function | Efficient protease with alanine specificity but only little elastolytic activity. [UniProt] |
| Cellular Localization | Cytoplasmic |
| Calculated MW | 29 kDa |
Images (1) Click the Picture to Zoom In
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ARG56185 anti-CELA3B / Elastase 3B antibody [CELA3B/1218] WB image
Western blot: 1) partial recombinant protein, 2) Panc-28, and 3) PANC1 cell lysates stained with ARG56185 anti-CELA3B / Elastase 3B antibody [CELA3B/1218].
