ARG54113
anti-CDX2 antibody
anti-CDX2 antibody for ICC/IF,Immunoprecipitation,Western blot and Human,Mouse,Monkey
Controls and Markers antibody; Developmental Biology antibody; Gene Regulation antibody
Overview
| Product Description | Mouse Monoclonal antibody recognizes CDX2 |
|---|---|
| Tested Reactivity | Hu, Ms, Mk |
| Tested Application | ICC/IF, IP, WB |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG2b |
| Target Name | CDX2 |
| Antigen Species | Human |
| Immunogen | Purified recombinant human CDX2 protein fragments expressed in E.coli |
| Conjugation | Un-conjugated |
| Alternate Names | CDX2/AS; CDX3; Homeobox protein CDX-2; CDX-3; Caudal-type homeobox protein 2 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purified |
| Buffer | PBS (pH 7.4), 0.2% Sodium azide and 50% Glycerol |
| Preservative | 0.2% Sodium azide |
| Stabilizer | 50% Glycerol |
| Concentration | 1.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | CDX2 |
| Gene Full Name | caudal type homeobox 2 |
| Background | Involved in the transcriptional regulation of multiple genes expressed in the intestinal epithelium.Important in broad range of functions from early differentiation to maintenance of the intestinal epithelial lining of both the small and large intestine. |
| Function | Involved in the transcriptional regulation of multiple genes expressed in the intestinal epithelium. Important in broad range of functions from early differentiation to maintenance of the intestinal epithelial lining of both the small and large intestine. [UniProt] |
| Cellular Localization | Nucleus |
| Research Area | Controls and Markers antibody; Developmental Biology antibody; Gene Regulation antibody |
| Calculated MW | 34 kDa |
| PTM | Phosphorylation of Ser-60 mediates the transactivation capacity. |
Images (3) Click the Picture to Zoom In
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ARG54113 anti-CDX2 antibody ICC/IF image
Immunofluorescence: 100% Methanol fixed (RT, 10 min) HeLa cells stained with ARG54113 anti-CDX2 antibody at 1:100 dilution. Left: primary antibody (green). Right: DAPI (blue).
Secondary antibody: ARG55393 Goat anti-Mouse IgG (H+L) antibody (FITC)
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ARG54113 anti-CDX2 antibody WB image
Western blot: 30 µg of NIH3T3 cell lysate stained with ARG54113 anti-CDX2 antibody at 1:1000 dilution.
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ARG54113 anti-CDX2 antibody IP image
Immunoprecipitation: HeLa cell lysate were immunoprecipitated and stained with ARG54113 anti-CDX2 antibody.
Customer's Feedback
Excellent
Review for anti-CDX2 antibody
Application：IF/ICC
Sample：HeLa
Fixation Buffer：100% Methanol
Fixation Time：10 min
Fixation Temperature：RT ºC
Permeabilization Buffer：0.1% Triton X-100
Primary Antibody Dilution Factor：1:100
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
Conjugation of Secondary Antibody：FITC
Excellent
Review for anti-CDX2 antibody
Application：WB
Sample：NIH3T3
Sample Loading Amount：30 µg
Primary Antibody Dilution Factor：1:1000
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
Average
Review for anti-CDX2 antibody
Application：WB
Sample：293T
Sample Loading Amount：30 µg
Primary Antibody Dilution Factor：1:1000
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
