ARG43921
anti-CDC42 antibody
anti-CDC42 antibody for ELISA,Flow cytometry,ICC/IF,Western blot and Human,Mouse,Rat
Overview
| Product Description | Rabbit Polyclonal antibody recognizes CDC42 |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | ELISA, FACS, ICC/IF, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Target Name | CDC42 |
| Antigen Species | Human |
| Immunogen | Human CDC42 recombinant protein |
| Expression System | E.coli |
| Conjugation | Un-conjugated |
| Protein Full Name | Cell division control protein 42 homolog |
| Alternate Names | CDC42; Cell Division Cycle 42; CDC42Hs; G25K; Cell Division Control Protein 42 Homolog; GTP Binding Protein, 25kDa; G25K GTP-Binding Protein; DJ224A6.1.1 (Cell Division Cycle 42 (GTP-Binding Protein, 25kD)); DJ224A6.1.2 (Cell Division Cycle 42 (GTP-Binding Protein, 25kD)); Cell Division Cycle 42 (GTP Binding Protein, 25kDa); Cell Division Cycle 42 (GTP-Binding Protein, 25kD); Small GTP Binding Protein CDC42; Growth-Regulating Protein; EC 3.6.5.2; TKS |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Buffer | 0.9% NaCl, 0.2% Na2HPO4 and 4% Trehalose. |
| Stabilizer | 4% Trehalose |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | CDC42 |
| Gene Full Name | Cell Division Cycle 42 |
| Background | The protein encoded by this gene is a small GTPase of the Rho-subfamily, which regulates signaling pathways that control diverse cellular functions including cell morphology, migration, endocytosis and cell cycle progression. This protein is highly similar to Saccharomyces cerevisiae Cdc 42, and is able to complement the yeast cdc42-1 mutant. The product of oncogene Dbl was reported to specifically catalyze the dissociation of GDP from this protein. This protein could regulate actin polymerization through its direct binding to Neural Wiskott-Aldrich syndrome protein (N-WASP), which subsequently activates Arp2/3 complex. Alternative splicing of this gene results in multiple transcript variants. Pseudogenes of this gene have been identified on chromosomes 3, 4, 5, 7, 8 and 20. |
| Function | Plasma membrane-associated small GTPase which cycles between an active GTP-bound and an inactive GDP-bound state. In active state binds to a variety of effector proteins to regulate cellular responses. Involved in epithelial cell polarization processes. Regulates the bipolar attachment of spindle microtubules to kinetochores before chromosome congression in metaphase. |
| Cellular Localization | Cell membrane, Cell projection, Cytoplasm, Cytoskeleton, Membrane |
| Calculated MW | 21 kDa |
| PTM | Glycoprotein, Lipoprotein, Methylation, Phosphoprotein, Prenylation |
Images (5) Click the Picture to Zoom In
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ARG43921 anti-CDC42 antibody ICC/IF image
Immunofluorescence: U2OS cells stained with ARG43921 anti-CDC42 antibody at 5 μg/ml dilution.
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ARG43921 anti-CDC42 antibody WB image
Western blot: A431 and 293T with ARG43921 anti-CDC42 antibody at 0.5 μg/mL dilution.
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ARG43921 anti-CDC42 antibody FACS image
Flow Cytometry: MCF-7 cells stained withARG43921 anti-CDC42 antibody (blue) at 1 μg/1x10^6 cells dilution.
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ARG43921 anti-CDC42 antibody WB image
Western blot: Rat brain and C6 with ARG43921 anti-CDC42 antibody at 0.5 μg/mL dilution.
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ARG43921 anti-CDC42 antibody WB image
Western blot: Mouse brain with ARG43921 anti-CDC42 antibody at 0.5 μg/mL dilution.
