ARG10887
anti-CDC27 antibody [AF 3.1]
anti-CDC27 antibody [AF 3.1] for ELISA,Flow cytometry,Immunohistochemistry,Immunoprecipitation,Western blot and Human,Mouse,Rat,Mammal,Xenopus laevis
Overview
| Product Description | Mouse Monoclonal antibody [AF 3.1] recognizes CDC27 |
|---|---|
| Tested Reactivity | Hu, Ms, Rat, Mamm, Xenopus laevis |
| Tested Application | ELISA, FACS, IHC, IP, WB |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | AF 3.1 |
| Isotype | IgG1 |
| Target Name | CDC27 |
| Antigen Species | Human |
| Immunogen | KLH-conjugated synthetic peptide around 10 aa (C-terminus) of Human Cdc27. |
| Conjugation | Un-conjugated |
| Alternate Names | HNUC; Cell division cycle protein 27 homolog; CDC27 homolog; NUC2; D0S1430E; H-NUC; Anaphase-promoting complex subunit 3; CDC27Hs; APC3; ANAPC3; D17S978E |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||||||
| Positive Control | HeLa cells. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified by affinity chromatography. |
| Buffer | PBS and 0.02% Sodium azide. |
| Preservative | 0.02% Sodium azide |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | CDC27 |
| Gene Full Name | cell division cycle 27 |
| Background | The protein encoded by this gene shares strong similarity with Saccharomyces cerevisiae protein Cdc27, and the gene product of Schizosaccharomyces pombe nuc 2. This protein is a component of the anaphase-promoting complex (APC), which is composed of eight protein subunits and is highly conserved in eukaryotic cells. This complex catalyzes the formation of cyclin B-ubiquitin conjugate, which is responsible for the ubiquitin-mediated proteolysis of B-type cyclins. The protein encoded by this gene and three other members of the APC complex contain tetratricopeptide (TPR) repeats, which are important for protein-protein interactions. This protein was shown to interact with mitotic checkpoint proteins including Mad2, p55CDC and BUBR1, and it may thus be involved in controlling the timing of mitosis. Alternative splicing of this gene results in multiple transcript variants. Related pseudogenes have been identified on chromosomes 2, 22 and Y. [provided by RefSeq, May 2014] |
| Function | Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains. [UniProt] |
| Calculated MW | 92 kDa |
| PTM | Phosphorylated. Phosphorylation on Ser-426 and Thr-446 occurs specifically during mitosis. [UniProt] |
