Overview

Product Description	Rabbit Polyclonal antibody recognizes CDC23
Tested Reactivity	Hu, Ms, Rat
Tested Application	ICC/IF, WB
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Target Name	CDC23
Antigen Species	Human
Immunogen	Synthetic peptide of Human CDC23.
Conjugation	Un-conjugated
Alternate Names	CUT23; Cell division cycle protein 23 homolog; ANAPC8; APC8; Anaphase-promoting complex subunit 8; Cyclosome subunit 8

Application Instructions

Application Suggestion	Tested Application Dilution ICC/IF 1:50 - 1:100 WB 1:500 - 1:2000
Application Note	* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control	HepG2
Observed Size	~ 65 kDa

Properties

Form	Liquid
Purification	Affinity purified.
Buffer	PBS (pH 7.4), 150 mM NaCl, 0.02% Sodium azide and 50% Glycerol.
Preservative	0.02% Sodium azide
Stabilizer	50% Glycerol
Storage Instruction	For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note	For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links	GeneID: 52563 Mouse CDC23 GeneID: 8697 Human CDC23 Swiss-port # Q8BGZ4 Mouse Cell division cycle protein 23 homolog Swiss-port # Q9UJX2 Human Cell division cycle protein 23 homolog
Gene Symbol	CDC23
Gene Full Name	cell division cycle 23
Background	The protein encoded by this gene shares strong similarity with Saccharomyces cerevisiae Cdc23, a protein essential for cell cycle progression through the G2/M transition. This protein is a component of anaphase-promoting complex (APC), which is composed of eight protein subunits and highly conserved in eukaryotic cells. APC catalyzes the formation of cyclin B-ubiquitin conjugate that is responsible for the ubiquitin-mediated proteolysis of B-type cyclins. This protein and 3 other members of the APC complex contain the TPR (tetratricopeptide repeat), a protein domain important for protein-protein interaction. [provided by RefSeq, Jul 2008]
Function	Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains. [UniProt]
Calculated MW	69 kDa
PTM	Phosphorylated. Phosphorylation on Thr-562 occurs specifically during mitosis. [UniProt]

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