ARG62951

anti-CD95 / Fas antibody [LT95]

anti-CD95 / Fas antibody [LT95] for Flow cytometry,IHC-Formalin-fixed paraffin-embedded sections and Human

Cell Biology and Cellular Response antibody; Cell Death antibody; Immune System antibody

Overview

Product Description Mouse Monoclonal antibody [LT95] recognizes CD95 / Fas
Tested Reactivity Hu
Tested Application FACS, IHC-P
Specificity The clone LT95 reacts with CD95 (Fas/APO-1), a 46 kDa single chain type I glycoprotein of the tumour necrosis factor/nerve growth factor (TNF/NGF) receptor superfamily, expressed on a variety of normal and neoplastic cells.
It seems that the antibody LT95 does not induce Fas mediated apoptosis, although it cross-blocks anti-Fas DX2 antibody that recognizes a functional epitope of Fas molecule.
Host Mouse
Clonality Monoclonal
Clone LT95
Isotype IgG1
Target Name CD95 / Fas
Antigen Species Human
Immunogen HUT-78 human T cell lymphoma cell line
Conjugation Un-conjugated
Alternate Names CD95; Apoptosis-mediating surface antigen FAS; FAS1; Tumor necrosis factor receptor superfamily member 6; ALPS1A; APT1; FASTM; CD antigen CD95; APO-1; TNFRSF6; FASLG receptor; Apo-1 antigen

Application Instructions

Application Suggestion
Tested Application Dilution
FACS1 - 12 µg/ml
IHC-PAssay-dependent
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control IHC-P: Tonsil

Properties

Form Liquid
Purification Purified from cell culture supernatant by protein-A affinity chromatography.
Purity > 95% (by SDS-PAGE)
Buffer PBS (pH 7.4) and 15 mM Sodium azide
Preservative 15 mM Sodium azide
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 355 Human FAS

Swiss-port # P25445 Human Tumor necrosis factor receptor superfamily member 6

Gene Symbol FAS
Gene Full Name Fas cell surface death receptor
Background CD95 (Fas, APO-1), a 46 kDa transmembrane glycoprotein, is a cell death receptor of the TNFR superfamily. Stimulation of CD95 results in aggregation of its intracellular death domains, formation of the death-inducing signaling complex (DISC) and activation of caspases. In type I cells caspase 3 is activated by high amounts of caspase 8 generated at the DISC, in type II cells low concentration of caspase 8 activates pathway leading to the release of cytochrome c from mitochondria and activation of caspase 3 by cytochom c. Besides its roles in induction of apoptosis, Fas also triggers pro-inflammatory cytokine responses.
Function Receptor for TNFSF6/FASLG. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. FAS-mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen-stimulated suicide of mature T-cells, or both. The secreted isoforms 2 to 6 block apoptosis (in vitro). [UniProt]
Research Area Cell Biology and Cellular Response antibody; Cell Death antibody; Immune System antibody
Calculated MW 38 kDa
PTM N- and O-glycosylated. O-glycosylated with core 1 or possibly core 8 glycans.

Images (1) Click the Picture to Zoom In

  • ARG62951 anti-CD95 / Fas antibody [LT95] FACS image

    Flow Cytometry: A) Jurkat cells, B) RAMOS cells, C) CEM human leukemia cells, and D) MOLT-4 cells stained with ARG62951 anti-CD95 / Fas antibody [LT95], followed by incubation with PE-labelled secondary antibody.

Clone References

Transformation by oncogenic RAS sensitizes human colon cells to TRAIL-induced apoptosis by up-regulating death receptor 4 and death receptor 5 through a MEK-dependent pathway.

Drosopoulos KG et al.
J Biol Chem.,  (2005)

publication_link

 

hr_line