ARG66139

anti-CD95 / Fas antibody (Biotin)

anti-CD95 / Fas antibody (Biotin) for ELISA,Western blot and Human

Overview

Product Description Biotin-conjugated Rabbit Polyclonal antibody recognizes CD95 / Fas
Tested Reactivity Hu
Tested Application ELISA, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name CD95 / Fas
Antigen Species Human
Immunogen E. coli derived recombinant Human CD95 / Fas.
(MRLSSKSVNA QVTDINSKGL ELRKTVTTVE TQNLEGLHHD GQFCHKPCPP GERKARDCTV NGDEPDCVPC QEGKEYTDKA HFSSKCRRCR LCDEGHGLEV EINCTRTQNT KCRCKPNFFC NSTVCEHCDP CTKCEHGIIK ECTLTSNTKC KEEGSRS)
Conjugation Biotin
Alternate Names CD95; Apoptosis-mediating surface antigen FAS; FAS1; Tumor necrosis factor receptor superfamily member 6; ALPS1A; APT1; FASTM; CD antigen CD95; APO-1; TNFRSF6; FASLG receptor; Apo-1 antigen

Application Instructions

Application Suggestion
Tested Application Dilution
ELISADirect: ~ 1.0 µg/ml
Sandwich: 0.25 - 1.0 µg/ml with ARG66138 as a capture antibody
WB0.1 - 0.2 µg/ml
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purified by affinity chromatography.
Buffer PBS (pH 7.2)
Concentration 1 mg/ml
Storage Instruction Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 355 Human FAS

Swiss-port # P25445 Human Tumor necrosis factor receptor superfamily member 6

Gene Symbol FAS
Gene Full Name Fas cell surface death receptor
Background The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor contains a death domain. It has been shown to play a central role in the physiological regulation of programmed cell death, and has been implicated in the pathogenesis of various malignancies and diseases of the immune system. The interaction of this receptor with its ligand allows the formation of a death-inducing signaling complex that includes Fas-associated death domain protein (FADD), caspase 8, and caspase 10. The autoproteolytic processing of the caspases in the complex triggers a downstream caspase cascade, and leads to apoptosis. This receptor has been also shown to activate NF-kappaB, MAPK3/ERK1, and MAPK8/JNK, and is found to be involved in transducing the proliferating signals in normal diploid fibroblast and T cells. Several alternatively spliced transcript variants have been described, some of which are candidates for nonsense-mediated mRNA decay (NMD). The isoforms lacking the transmembrane domain may negatively regulate the apoptosis mediated by the full length isoform. [provided by RefSeq, Mar 2011]
Function Receptor for TNFSF6/FASLG. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. FAS-mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen-stimulated suicide of mature T-cells, or both. The secreted isoforms 2 to 6 block apoptosis (in vitro). [UniProt]
Highlight Related products:
CD95 antibodies; CD95 ELISA Kits; Anti-Rabbit IgG secondary antibodies;
Calculated MW 38 kDa
PTM N- and O-glycosylated. O-glycosylated with core 1 or possibly core 8 glycans.

Images (4) Click the Picture to Zoom In

  • ARG66139 anti-CD95 / Fas antibody (Biotin) WB image

    Western blot: 250 - 0.24 ng of Human sFas Receptor stained with ARG66139 anti-CD95 / Fas antibody (Biotin), under non-reducing conditions.

  • ARG66139 anti-CD95 / Fas antibody (Biotin) standard curve image

    Direct ELISA: ARG66139 anti-CD95 / Fas antibody (Biotin) at ~ 1.0 µg/ml results of a typical standard run with optical density reading at 405 - 650 nm.

  • ARG66139 anti-CD95 / Fas antibody (Biotin) WB image

    Western blot: 250 - 0.24 ng of Human sFas Receptor stained with ARG66139 anti-CD95 / Fas antibody (Biotin), under reducing conditions.

  • ARG66139 anti-CD95 / Fas antibody (Biotin) standard curve image

    Sandwich ELISA: ARG66139 anti-CD95 / Fas antibody (Biotin) as a detection antibody at 0.25 - 1.0 µg/ml combined with ARG66138 anti-CD95 / Fas antibody as a capture antibody. Results of a typical standard run with optical density reading at 405 - 650 nm.