ARG66138

anti-CD95 / Fas antibody

anti-CD95 / Fas antibody for ELISA,Western blot and Human

Overview

Product Description Rabbit Polyclonal antibody recognizes CD95 / Fas
Tested Reactivity Hu
Tested Application ELISA, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name CD95 / Fas
Antigen Species Human
Immunogen E. coli derived recombinant Human CD95 / Fas.
(MRLSSKSVNA QVTDINSKGL ELRKTVTTVE TQNLEGLHHD GQFCHKPCPP GERKARDCTV NGDEPDCVPC QEGKEYTDKA HFSSKCRRCR LCDEGHGLEV EINCTRTQNT KCRCKPNFFC NSTVCEHCDP CTKCEHGIIK ECTLTSNTKC KEEGSRS)
Conjugation Un-conjugated
Alternate Names CD95; Apoptosis-mediating surface antigen FAS; FAS1; Tumor necrosis factor receptor superfamily member 6; ALPS1A; APT1; FASTM; CD antigen CD95; APO-1; TNFRSF6; FASLG receptor; Apo-1 antigen

Application Instructions

Application Suggestion
Tested Application Dilution
ELISASandwich: 0.5 - 2.0 µg/ml with ARG66139 as a detection antibody
WB0.1 - 0.2 µg/ml
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Affinity purification with immunogen.
Buffer PBS (pH 7.2)
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 355 Human FAS

Swiss-port # P25445 Human Tumor necrosis factor receptor superfamily member 6

Gene Symbol FAS
Gene Full Name Fas cell surface death receptor
Background The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor contains a death domain. It has been shown to play a central role in the physiological regulation of programmed cell death, and has been implicated in the pathogenesis of various malignancies and diseases of the immune system. The interaction of this receptor with its ligand allows the formation of a death-inducing signaling complex that includes Fas-associated death domain protein (FADD), caspase 8, and caspase 10. The autoproteolytic processing of the caspases in the complex triggers a downstream caspase cascade, and leads to apoptosis. This receptor has been also shown to activate NF-kappaB, MAPK3/ERK1, and MAPK8/JNK, and is found to be involved in transducing the proliferating signals in normal diploid fibroblast and T cells. Several alternatively spliced transcript variants have been described, some of which are candidates for nonsense-mediated mRNA decay (NMD). The isoforms lacking the transmembrane domain may negatively regulate the apoptosis mediated by the full length isoform. [provided by RefSeq, Mar 2011]
Function Receptor for TNFSF6/FASLG. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. FAS-mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen-stimulated suicide of mature T-cells, or both. The secreted isoforms 2 to 6 block apoptosis (in vitro). [UniProt]
Calculated MW 38 kDa
PTM N- and O-glycosylated. O-glycosylated with core 1 or possibly core 8 glycans.

Images (3) Click the Picture to Zoom In

  • ARG66138 anti-CD95 / Fas antibody WB image

    Western blot: 250 - 0.24 ng of Human sFas Receptor stained with ARG66138 anti-CD95 / Fas antibody, under non-reducing conditions.

  • ARG66138 anti-CD95 / Fas antibody standard curve image

    Sandwich ELISA: ARG66138 anti-CD95 / Fas antibody as a capture antibody at 0.5 - 2.0 µg/ml combined with ARG66139 anti-CD95 / Fas antibody (Biotin) as a detection antibody. Results of a typical standard run with optical density reading at 405 - 650 nm.

  • ARG66138 anti-CD95 / Fas antibody WB image

    Western blot: 250 - 0.24 ng of Human sFas Receptor stained with ARG66138 anti-CD95 / Fas antibody, under reducing conditions.