ARG64992

anti-CD95 / Fas antibody

anti-CD95 / Fas antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human

Cell Biology and Cellular Response antibody; Cell Death antibody; Immune System antibody

Overview

Product Description Goat Polyclonal antibody recognizes CD95 / Fas
Tested Reactivity Hu
Tested Application IHC-P, WB
Specificity This antibody is expected to recognize reported isoforms 1 (NP_000034.1), 2 (NP_690610.1) and 3 (NP_690611.1).
Host Goat
Clonality Polyclonal
Isotype IgG
Target Name CD95 / Fas
Antigen Species Human
Immunogen KTCRKHRKENQGSH
Conjugation Un-conjugated
Alternate Names CD95; Apoptosis-mediating surface antigen FAS; FAS1; Tumor necrosis factor receptor superfamily member 6; ALPS1A; APT1; FASTM; CD antigen CD95; APO-1; TNFRSF6; FASLG receptor; Apo-1 antigen

Application Instructions

Application Suggestion
Tested Application Dilution
IHC-P3 - 5 µg/ml
WB0.1 - 0.3 µg/ml
Application Note WB: Recommend incubate at RT for 1h.
IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0).
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Buffer Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA
Preservative 0.02% Sodium azide
Stabilizer 0.5% BSA
Concentration 0.5 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 355 Human FAS

Swiss-port # P25445 Human Tumor necrosis factor receptor superfamily member 6

Background The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor contains a death domain. It has been shown to play a central role in the physiological regulation of programmed cell death, and has been implicated in the pathogenesis of various malignancies and diseases of the immune system. The interaction of this receptor with its ligand allows the formation of a death-inducing signaling complex that includes Fas-associated death domain protein (FADD), caspase 8, and caspase 10. The autoproteolytic processing of the caspases in the complex triggers a downstream caspase cascade, and leads to apoptosis. This receptor has been also shown to activate NF-kappaB, MAPK3/ERK1, and MAPK8/JNK, and is found to be involved in transducing the proliferating signals in normal diploid fibroblast and T cells. Several alternatively spliced transcript variants have been described, some of which are candidates for nonsense-mediated mRNA decay (NMD). The isoforms lacking the transmembrane domain may negatively regulate the apoptosis mediated by the full length isoform. [provided by RefSeq, Mar 2011]
Research Area Cell Biology and Cellular Response antibody; Cell Death antibody; Immune System antibody
Calculated MW 38 kDa
PTM N- and O-glycosylated. O-glycosylated with core 1 or possibly core 8 glycans.

Images (3) Click the Picture to Zoom In

  • ARG64992 anti-FAS / CD95 antibody WB image

    Western Blot: MOLT4 lysate (35 µg protein in RIPA buffer) stained with ARG64992 anti-FAS / CD95 antibody at 0.1 µg/ml dilution.

  • ARG64992 anti-CD95 / Fas antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Human tonsil tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG64992 anti-CD95 / Fas antibody at 3.75 µg/ml dilution followed by AP-staining.

  • ARG64992 anti-CD95 / Fas antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Human small intestine tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG64992 anti-CD95 / Fas antibody at 3.75 µg/ml dilution followed by AP-staining.