ARG57542
anti-CD94 / KLRD1 antibody [13E3]
anti-CD94 / KLRD1 antibody [13E3] for Flow cytometry,Western blot and Human
Overview
| Product Description | Mouse Monoclonal antibody [13E3] recognizes CD94 / KLRD1 |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | FACS, WB |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | 13E3 |
| Isotype | IgG1, kappa |
| Target Name | CD94 / KLRD1 |
| Antigen Species | Human |
| Immunogen | Recombinant Human CD94 / KLRD1 (aa. 32-179) purified from E. coli |
| Conjugation | Un-conjugated |
| Alternate Names | KP43; CD antigen CD94; CD94; Natural killer cells antigen CD94; Killer cell lectin-like receptor subfamily D member 1; NK cell receptor |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein A. |
| Buffer | PBS (pH 7.4), 0.02% Sodium azide and 10% Glycerol. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 10% Glycerol |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | KLRD1 |
| Gene Full Name | killer cell lectin-like receptor subfamily D, member 1 |
| Background | Natural killer (NK) cells are a distinct lineage of lymphocytes that mediate cytotoxic activity and secrete cytokines upon immune stimulation. Several genes of the C-type lectin superfamily, including members of the NKG2 family, are expressed by NK cells and may be involved in the regulation of NK cell function. KLRD1 (CD94) is an antigen preferentially expressed on NK cells and is classified as a type II membrane protein because it has an external C terminus. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
| Function | Plays a role as a receptor for the recognition of MHC class I HLA-E molecules by NK cells and some cytotoxic T-cells. [UniProt] |
| Calculated MW | 21 kDa |
Images (3) Click the Picture to Zoom In
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ARG57542 anti-CD94 / KLRD1 antibody [13E3] FACS image
Flow Cytometry: PBMC cells stained with ARG57542 anti-CD94 / KLRD1 antibody [13E3] at 2 - 5 µg/10^6 cells (red). Secondary Ab: Goat anti-Mouse IgG antibody (Alexa fluor 488). Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray).
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ARG57542 anti-CD94 / KLRD1 antibody [13E3] WB image
Western blot: 40 µg of K562, MCF7 and A549 cell lysates stained with ARG57542 anti-CD94 / KLRD1 antibody [13E3] at 1:1000 dilution.
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ARG57542 anti-CD94 / KLRD1 antibody [13E3] WB image
Western blot: 40 µg of NK-92 cell lysate stained with ARG57542 anti-CD94 / KLRD1 antibody [13E3] at 1:1000 dilution.
