ARG41919
anti-CD90 / Thy 1 antibody
anti-CD90 / Thy 1 antibody for Flow cytometry,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Mouse,Rat
Overview
| Product Description | Rabbit Polyclonal antibody recognizes CD90 / Thy 1 |
|---|---|
| Tested Reactivity | Ms, Rat |
| Tested Application | FACS, IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | CD90 / Thy 1 |
| Antigen Species | Mouse |
| Immunogen | Recombinant protein corresponding to Q20-C131 of Mouse CD90 / Thy 1. |
| Conjugation | Un-conjugated |
| Alternate Names | Thy-1 membrane glycoprotein; Thy-1 antigen; CD antigen CD90; CDw90; CD90 |
Application Instructions
| Application Suggestion |
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| Application Note | IHC-P: Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0, epitope retrieval solution) for 20 min. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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| Observed Size | ~ 27 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification with immunogen. |
| Buffer | 0.2% Na2HPO4, 0.9% NaCl, 0.05% Sodium azide and 4% Trehalose. |
| Preservative | 0.05% Sodium azide |
| Stabilizer | 4% Trehalose |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | THY1 |
| Gene Full Name | Thy-1 cell surface antigen |
| Background | This gene encodes a cell surface glycoprotein and member of the immunoglobulin superfamily of proteins. The encoded protein is involved in cell adhesion and cell communication in numerous cell types, but particularly in cells of the immune and nervous systems. The encoded protein is widely used as a marker for hematopoietic stem cells. This gene may function as a tumor suppressor in nasopharyngeal carcinoma. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2015] |
| Function | May play a role in cell-cell or cell-ligand interactions during synaptogenesis and other events in the brain. [UniProt] |
| Cellular Localization | Cell membrane; Lipid-anchor, GPI-anchor. [UniProt] |
| Calculated MW | 18 kDa |
Images (5) Click the Picture to Zoom In
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ARG41919 anti-CD90 / Thy 1 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Mouse spleen tissue. Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0, epitope retrieval solution) for 20 min. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG41919 anti-CD90 / Thy 1 antibody at 1 µg/ml dilution, overnight at 4°C.
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ARG41919 anti-CD90 / Thy 1 antibody WB image
Western blot: 50 µg of samples under reducing conditions. Mouse brain, Mouse thymus, Mouse lung and Rat thymus lysates stained with ARG41919 anti-CD90 / Thy 1 antibody at 0.5 µg/ml dilution, overnight at 4°C.
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ARG41919 anti-CD90 / Thy 1 antibody FACS image
Flow Cytometry: YAC-1 cells were blocked with 10% normal goat serum and then stained with ARG41919 anti-CD90 / Thy 1 antibody (blue) at 1 µg/10^6 cells for 30 min at 20°C, followed by incubation with DyLight®488 labelled secondary antibody. Isotype control antibody (green) was Rabbit IgG (1 µg/10^6 cells) used under the same conditions. Unlabelled sample (red) was also used as a control.
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ARG41919 anti-CD90 / Thy 1 antibody FACS image
Flow Cytometry: Neuro-2a cells were blocked with 10% normal goat serum and then stained with ARG41919 anti-CD90 / Thy 1 antibody (blue) at 1 µg/10^6 cells for 30 min at 20°C, followed by incubation with DyLight®488 labelled secondary antibody. Isotype control antibody (green) was Rabbit IgG (1 µg/10^6 cells) used under the same conditions. Unlabelled sample (red) was also used as a control.
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ARG41919 anti-CD90 / Thy 1 antibody FACS image
Flow Cytometry: SP2-0 cells were blocked with 10% normal goat serum and then stained with ARG41919 anti-CD90 / Thy 1 antibody (blue) at 1 µg/10^6 cells for 30 min at 20°C, followed by incubation with DyLight®488 labelled secondary antibody. Isotype control antibody (green) was Rabbit IgG (1 µg/10^6 cells) used under the same conditions. Unlabelled sample (red) was also used as a control.
