ARG62945
anti-CD9 antibody [IVA50]
anti-CD9 antibody [IVA50] for Flow cytometry,Functional study,Immunoprecipitation,Western blot and Bovine
Cell Biology and Cellular Response antibody; Developmental Biology antibody; Immune System antibody
Overview
| Product Description | Mouse Monoclonal antibody [IVA50] recognizes CD9 |
|---|---|
| Tested Reactivity | Bov |
| Tested Application | FACS, FuncSt, IP, WB |
| Specificity | The clone IVA50 reacts with CD9 antigen (bovine), a 24 kDa single transmembrane polypeptide expressed on platelets, monocytes, pre-B lymphocytes, granulocytes and activated T lymphocytes. |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | IVA50 |
| Isotype | IgG2 |
| Target Name | CD9 |
| Immunogen | Bovine thrombocytes. |
| Conjugation | Un-conjugated |
| Alternate Names | Leukocyte antigen MIC3; BTCC-1; TSPAN-29; MIC3; Tetraspanin-29; p24; Cell growth-inhibiting gene 2 protein; CD9 antigen; MRP-1; DRAP-27; 5H9 antigen; CD antigen CD9; TSPAN29; Motility-related protein; Tspan-29 |
Application Instructions
| Application Suggestion |
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| Application Note | Functional studies: The clone IVA50 activates Bovine thrombocytes. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from ascites by protein-A affinity chromatography. |
| Purity | > 95% (by SDS-PAGE) |
| Buffer | PBS (pH 7.4) and 15 mM Sodium azide |
| Preservative | 15 mM Sodium azide |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | CD9 |
| Gene Full Name | CD9 molecule |
| Background | This gene encodes a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Tetraspanins are cell surface glycoproteins with four transmembrane domains that form multimeric complexes with other cell surface proteins. The encoded protein functions in many cellular processes including differentiation, adhesion, and signal transduction, and expression of this gene plays a critical role in the suppression of cancer cell motility and metastasis. [provided by RefSeq, Jan 2011] |
| Function | Involved in platelet activation and aggregation. Regulates paranodal junction formation. Involved in cell adhesion, cell motility and tumor metastasis. Required for sperm-egg fusion (By similarity). [UniProt] |
| Research Area | Cell Biology and Cellular Response antibody; Developmental Biology antibody; Immune System antibody |
| Calculated MW | 25 kDa |
| PTM | Palmitoylated at a low, basal level in unstimulated platelets. The level of palmitoylation increases when platelets are activated by thrombin (in vitro). The protein exists in three forms with molecular masses between 22 and 27 kDa, and is known to carry covalently linked fatty acids. |
Images (3) Click the Picture to Zoom In
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ARG62945 anti-CD9 antibody [IVA50] IP image
Immunoprecipitation: Biotin-labeled human platelets lysates was immunoprecipitated with 1) ARG62945 anti-CD9 antibody [IVA50], 2) anti-Human CD9 control antibody, and 3) negative control.
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ARG62945 anti-CD9 antibody [IVA50] FACS image
Flow Cytometry: Bovine peripheral whole blood stained with ARG62945 anti-CD9 antibody [IVA50] at 10 µg/ml dilution, followed by APC-conjugated Goat anti-Mouse antibody.
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ARG62945 anti-CD9 antibody [IVA50] FACS image
Flow Cytometry: Separation of bovine CD9 positive thrombocytes (red-filled) from lymphocytes (black-dashed). Bovine peripheral whole blood stained with ARG62945 anti-CD9 antibody [IVA50] at 10 µg/ml dilution, followed by APC-conjugated Goat anti-Mouse antibody.
Clone References
Exosomes from bone marrow dendritic cells pulsed with diphtheria toxoid preferentially induce type 1 antigen-specific IgG responses in naive recipients in the absence of free antigen.
ELISA / Cow
A set of monoclonal antibodies specific for bovine cell surface molecule CD9.
Nomenclature and characterization of leukocyte differentiation antigens in ruminants.
