ARG65537
anti-CD88 / C5AR1 antibody [S5/1]
anti-CD88 / C5AR1 antibody [S5/1] for CyTOF®-candidate,Flow cytometry,IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation,Western blot and Human,Bovine,Ferret,Rabbit
Cell Biology and Cellular Response antibody; Immune System antibody; Metabolism antibody; Signaling Transduction antibody
Overview
| Product Description | Mouse Monoclonal antibody [S5/1] recognizes CD88 / C5AR1 |
|---|---|
| Tested Reactivity | Hu, Bov, Frt, Rb |
| Tested Application | CyTOF®-candidate, FACS, IHC-P, IP, WB |
| Specificity | The mouse monoclonal antibody S5/1 recognizes the CD88 protein, a 43 kDa receptor of C5a component of the complement cascade. |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | S5/1 |
| Isotype | IgG2a |
| Target Name | CD88 / C5AR1 |
| Antigen Species | Human |
| Immunogen | Recombinant N-terminal peptide (Asp15-Asp27) of human C5aR |
| Conjugation | Un-conjugated |
| Alternate Names | CD88; C5R1; C5AR; CD antigen CD88; C5a anaphylatoxin chemotactic receptor 1; C5a anaphylatoxin chemotactic receptor; C5A; C5aR; C5a-R |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from ascites by protein-A affinity chromatography. |
| Purity | > 95% (by SDS-PAGE) |
| Buffer | PBS (pH 7.4) and 15 mM Sodium azide |
| Preservative | 15 mM Sodium azide |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P21730 Human C5a anaphylatoxin chemotactic receptor 1 |
|---|---|
| Gene Symbol | C5AR1 |
| Gene Full Name | complement component 5a receptor 1 |
| Background | CD88 / C5aR is a G protein-coupled seven membrane-spanning protein serving as a receptor for C5a component of the complement cascade, and is expressed mainly by monocytes, macrophages, neutrophils, eosinophils, and mast cells, but also e.g. by hepatocytes, glial cells, vascular endothelial cells, or cardiomyocytes. The binding of C5a to CD88 is associated with inflammatory response, including superoxide anion production, chemotaxis, and increased production of acute phase proteins. Expression of CD88 on synovial mast cells and their C5a-mediated degranulation plays a role in pathogenesis of rheumatoid arthritis. |
| Function | Receptor for the chemotactic and inflammatory peptide anaphylatoxin C5a (PubMed:1847994, PubMed:8182049, PubMed:7622471, PubMed:9553099, PubMed:10636859, PubMed:15153520). The ligand interacts with at least two sites on the receptor: a high-affinity site on the extracellular N-terminus, and a second site in the transmembrane region which activates downstream signaling events (PubMed:8182049, PubMed:7622471, PubMed:9553099). Receptor activation stimulates chemotaxis, granule enzyme release, intracellular calcium release and superoxide anion production (PubMed:10636859, PubMed:15153520). [UniProt] |
| Highlight | Related products: CD88 antibodies; Anti-Mouse IgG secondary antibodies; Related news: CyTOF-candidate Antibodies |
| Research Area | Cell Biology and Cellular Response antibody; Immune System antibody; Metabolism antibody; Signaling Transduction antibody |
| Calculated MW | 39 kDa |
| PTM | Sulfation plays a critical role in the association of C5aR with C5a, but no significant role in the ability of the receptor to transduce a signal and mobilize calcium in response to a small a small peptide agonist (PubMed:11342590). Sulfation at Tyr-14 is important for CHIPS binding (PubMed:21706042). Phosphorylated on serine residues in response to C5a binding, resulting in internalization of the receptor and short-term desensitization to the ligand. The key residues involved in this process are Ser-334 and Ser-338. |
Clone References
C5a and its receptors in human anti-neutrophil cytoplasmic antibody (ANCA)-associated vasculitis.
IHC-P / Human
C5a mediates peripheral blood neutrophil dysfunction in critically ill patients.
Probing the human receptor for C5a anaphylatoxin with site-directed antibodies. Identification of a potential ligand binding site on the NH2-terminal domain.
