ARG22560

anti-CD88 / C5AR1 antibody [20/70] (PE)

anti-CD88 / C5AR1 antibody [20/70] (PE) for Flow cytometry and Mouse

Overview

Product Description PE-conjugated Rat Monoclonal antibody [20/70] recognizes CD88 / C5AR1
This antibody recognizes murine CD88, also known as C5a anaphylatoxin chemotactic receptor 1 (C5aR1), a member of the G-protein coupled receptor 1 family. CD88 is a ~45kDa multi pass membrane protein and functions as a receptor for the complement component C5a, a potent proinflammatory molecule and a chemoattractant for neutrophils to sites of infection. In mouse, CD88 is expressed on granulocytes, monocytes and macrophages but not on resting or stimulated lymphocytes. Clone 20/70 has been reported to block the binding of the C5a to murine CD88, (Soruri et al. 2003).
Tested Reactivity Ms
Tested Application FACS
Host Rat
Clonality Monoclonal
Clone 20/70
Isotype IgG2b
Target Name CD88 / C5AR1
Antigen Species Mouse
Immunogen RBL-2H3 transfected cells expressing murine CD88 / C5aR.
Conjugation PE
Alternate Names CD88; C5R1; C5AR; CD antigen CD88; C5a anaphylatoxin chemotactic receptor 1; C5a anaphylatoxin chemotactic receptor; C5A; C5aR; C5a-R

Application Instructions

Application Suggestion
Tested Application Dilution
FACS1:50 - 1:100
Application Note FACS: Use 10ul of the suggested working dilution to label 10^6 cells in 100ul.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purification with Protein G.
Buffer PBS, 0.09% Sodium azide, 1% BSA and 5% Sucrose.
Preservative 0.09% Sodium azide
Stabilizer 1% BSA and 5% Sucrose
Storage Instruction Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 12273 Mouse C5AR1

Swiss-port # P30993 Mouse C5a anaphylatoxin chemotactic receptor 1

Gene Symbol C5ar1
Gene Full Name complement component 5a receptor 1
Function Receptor for the chemotactic and inflammatory peptide anaphylatoxin C5a. The ligand interacts with at least two sites on the receptor: a high-affinity site on the extracellular N-terminus, and a second site in the transmembrane region which activates downstream signaling events. Receptor activation stimulates chemotaxis, granule enzyme release, intracellular calcium release and superoxide anion production. [UniProt]
Calculated MW 39 kDa
PTM Sulfation plays a critical role in the association of C5aR with C5a, but no significant role in the ability of the receptor to transduce a signal and mobilize calcium in response to a small a small peptide agonist (PubMed:11342590). Sulfation at Tyr-14 is important for CHIPS binding (PubMed:21706042).
Phosphorylated on serine residues in response to C5a binding, resulting in internalization of the receptor and short-term desensitization to the ligand. The key residues involved in this process are Ser-334 and Ser-338.