ARG62938

anti-CD86 antibody [GL-1]

anti-CD86 antibody [GL-1] for CyTOF®-candidate,Flow cytometry,Functional study,ICC/IF,IHC-Frozen sections,Immunoprecipitation and Mouse

Developmental Biology antibody; Immune System antibody; Microbiology and Infectious Disease antibody

Overview

Product Description Rat Monoclonal antibody [GL-1] recognizes CD86
Tested Reactivity Ms
Tested Application CyTOF®-candidate, FACS, FuncSt, ICC/IF, IHC-Fr, IP
Specificity The clone GL-1 reacts with CD86 (B7-2), a 70-80 kDa type I transmembrane glycoprotein of immunoglobulin supergene family, expressed on professional antigen-presenting cells, such as dendritic cells, macrophages or activated B lymphocytes.
Host Rat
Clonality Monoclonal
Clone GL-1
Isotype IgG2a
Target Name CD86
Antigen Species Mouse
Immunogen LPS-activated CBA/Cs mouse splenic B cells.
Conjugation Un-conjugated
Alternate Names B70; B7.2; LAB72; CD antigen CD86; B7-2; FUN-1; CD28LG2; T-lymphocyte activation antigen CD86; CTLA-4 counter-receptor B7.2; Activation B7-2 antigen; BU63

Application Instructions

Application Suggestion
Tested Application Dilution
CyTOF®-candidateAssay-dependent
FACS2 µg/ml
FuncStAssay-dependent
ICC/IFAssay-dependent
IHC-FrAssay-dependent
IPAssay-dependent
Application Note Functional studies: Blocking.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control FACS: Mouse splenocytes

Properties

Form Liquid
Purification Purified from hybridoma culture supernatant by protein-G affinity chromatography.
Purity > 95% (by SDS-PAGE)
Buffer PBS (pH 7.4) and 15 mM Sodium azide
Preservative 15 mM Sodium azide
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 12524 Mouse CD86

Swiss-port # P42082 Mouse T-lymphocyte activation antigen CD86

Gene Symbol Cd86
Gene Full Name CD86 antigen
Background CD80 (B7-1) and CD86 (B7-2) are ligands of T cell critical costimulatory molecule CD28 and of an inhibitory receptor CTLA-4 (CD152). The both B7 molecules are expressed on professional antigen-presenting cells and are essential for T cell activation, the both molecules can also substitute for each other in this process. The question what are the differences in CD80 and CD86 competency has not been fully elucidated yet; there are still conflicts in results about their respective roles in initiation or sustaining of the T cell immune response.
Function Receptor involved in the costimulatory signal essential for T-lymphocyte proliferation and interleukin-2 production, by binding CD28 or CTLA-4. May play a critical role in the early events of T-cell activation and costimulation of naive T-cells, such as deciding between immunity and anergy that is made by T-cells within 24 hours after activation. [UniProt]
Highlight Related products:
CD86 antibodies; CD86 ELISA Kits; Anti-Rat IgG secondary antibodies;
Related news:
CyTOF-candidate Antibodies
Research Area Developmental Biology antibody; Immune System antibody; Microbiology and Infectious Disease antibody
Calculated MW 38 kDa
PTM Polyubiquitinated; which is promoted by MARCH8 and results in endocytosis and lysosomal degradation.

Images (2) Click the Picture to Zoom In

  • ARG62938 anti-CD86 antibody [GL-1] FACS image

    Flow Cytometry: Murine peritoneal fluid cells suspension stained with ARG62938 anti-CD86 antibody [GL-1] at 0.6 µg/ml dilution, followed by APC-conjugated Donkey anti-Rat antibody.

  • ARG62938 anti-CD86 antibody [GL-1] FACS image

    Flow Cytometry: Separation of murine CD86 positive myeloid cells (red-filled) from murine CD86 negative lymphocytes (black-dashed). Murine peritoneal fluid cells suspension stained with ARG62938 anti-CD86 antibody [GL-1] at 0.6 µg/ml dilution, followed by APC-conjugated Donkey anti-Rat antibody.

Clone References

Inflammatory cascades mediate synapse elimination in spinal cord compression.

IHC-FoFr / Mouse

Takano M et al.
J Neuroinflammation.,  (2014)

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Live single cell functional phenotyping in droplet nano-liter reactors.

Konry T et al.
Sci Rep.,  (2013)

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Intrarenal antigens activate CD4+ cells via co-stimulatory signals from dendritic cells.

Edgtton KL et al.
J Am Soc Nephrol.,  (2008)

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Autoimmune diabetes is suppressed by transfer of proinsulin-encoding Gr-1+ myeloid progenitor cells that differentiate in vivo into resting dendritic cells.

Steptoe RJ et al.
Diabetes.,  (2005)

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