ARG58026

anti-CD86 antibody [C86/1146]

anti-CD86 antibody [C86/1146] for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human

Overview

Product Description Mouse Monoclonal antibody [C86/1146] recognizes CD86
Tested Reactivity Hu
Predict Reactivity Ms, Rat
Tested Application ICC/IF, IHC-P, WB
Host Mouse
Clonality Monoclonal
Clone C86/1146
Isotype IgG1, kappa
Target Name CD86
Antigen Species Human
Immunogen Recombinant full-length protein of Human CD86.
Conjugation Un-conjugated
Alternate Names B70; B7.2; LAB72; CD antigen CD86; B7-2; FUN-1; CD28LG2; T-lymphocyte activation antigen CD86; CTLA-4 counter-receptor B7.2; Activation B7-2 antigen; BU63

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IF2 - 5 µg/ml
IHC-P2 - 5 µg/ml
WB1 - 2 µg/ml
Application Note IHC-P: Antigen Retrieval: Boil tissue section in 10 mM Tris buffer with 1 mM EDTA (pH 9.0) for 10-20 min, followed by cooling at RT for 20 min.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purification with Protein G.
Buffer PBS, 0.05% Sodium azide and 0.1 mg/ml BSA.
Preservative 0.05% Sodium azide
Stabilizer 0.1 mg/ml BSA
Concentration 0.2 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 942 Human CD86

Swiss-port # P42081 Human T-lymphocyte activation antigen CD86

Gene Symbol CD86
Gene Full Name CD86 molecule
Background This gene encodes a type I membrane protein that is a member of the immunoglobulin superfamily. This protein is expressed by antigen-presenting cells, and it is the ligand for two proteins at the cell surface of T cells, CD28 antigen and cytotoxic T-lymphocyte-associated protein 4. Binding of this protein with CD28 antigen is a costimulatory signal for activation of the T-cell. Binding of this protein with cytotoxic T-lymphocyte-associated protein 4 negatively regulates T-cell activation and diminishes the immune response. Alternative splicing results in several transcript variants encoding different isoforms.[provided by RefSeq, May 2011]
Function Receptor involved in the costimulatory signal essential for T-lymphocyte proliferation and interleukin-2 production, by binding CD28 or CTLA-4. May play a critical role in the early events of T-cell activation and costimulation of naive T-cells, such as deciding between immunity and anergy that is made by T-cells within 24 hours after activation. Isoform 2 interferes with the formation of CD86 clusters, and thus acts as a negative regulator of T-cell activation. [UniProt]
Calculated MW 38 kDa
PTM Polyubiquitinated; which is promoted by MARCH8 and results in endocytosis and lysosomal degradation. [UniProt]

Images (3) Click the Picture to Zoom In

  • ARG58026 anti-CD86 antibody [C86/1146] ICC/IF image

    Immunofluorescence: Raji cells stained with ARG58026 anti-CD86 antibody [C86/1146] (green). Reddot (red) for nuclear staining.

  • ARG58026 anti-CD86 antibody [C86/1146] IHC-P image

    Immunohistochemistry: Formalin-fixed and paraffin-embedded Human melanoma stained with ARG58026 anti-CD86 antibody [C86/1146].

  • ARG58026 anti-CD86 antibody [C86/1146] WB image

    Western blot: Daudi cell lysate stained with ARG58026 anti-CD86 antibody [C86/1146].