ARG23462

anti-CD86 antibody [24F]

anti-CD86 antibody [24F] for Flow cytometry,IHC-Frozen sections,Immunoprecipitation and Rat

Overview

Product Description Mouse Monoclonal antibody [24F] recognizes CD86
Mouse anti Rat CD86 antibody, clone 24F recognizes rat CD86, otherwise known as B7-2, a type I transmembrane protein and member of the Ig superfamily, which acts as a ligand for both CD28 and CD152 (CTLA-4), and is primarily expressed on antigen presenting cells (APCs) including dendritic cells, and also on germinal centre B cells and macrophages. Like CD80, CD86 is an accessory molecule which functions in the CD28-CD80/CD86 co-stimulatory pathway, vital for T cell activation, crosstalk between T and B cells, and Th2-mediated Ig production. Mouse anti Rat CD86 antibody, clone 24F has been shown to block the co-stimulatory activity of rat CD86 (Maeda et al. 1997).
Tested Reactivity Rat
Tested Application FACS, IHC-Fr, IP
Host Mouse
Clonality Monoclonal
Clone 24F
Isotype IgG1
Target Name CD86
Antigen Species Rat
Immunogen HTLV-1 transformed Lewis-S1 cells.
Conjugation Un-conjugated
Alternate Names B70; B7.2; LAB72; CD antigen CD86; B7-2; FUN-1; CD28LG2; T-lymphocyte activation antigen CD86; CTLA-4 counter-receptor B7.2; Activation B7-2 antigen; BU63

Application Instructions

Application Suggestion
Tested Application Dilution
FACSNeat
IHC-FrAssay-dependent
IPAssay-dependent
Application Note FACS: Use 10 µl of the suggested working dilution to label 10^6 cells in 100 µl.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purification with Protein G.
Buffer PBS and 0.09% Sodium azide.
Preservative 0.09% Sodium azide
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Gene Symbol CD86
Gene Full Name CD86 molecule
Background This gene encodes a type I membrane protein that is a member of the immunoglobulin superfamily. This protein is expressed by antigen-presenting cells, and it is the ligand for two proteins at the cell surface of T cells, CD28 antigen and cytotoxic T-lymphocyte-associated protein 4. Binding of this protein with CD28 antigen is a costimulatory signal for activation of the T-cell. Binding of this protein with cytotoxic T-lymphocyte-associated protein 4 negatively regulates T-cell activation and diminishes the immune response. Alternative splicing results in several transcript variants encoding different isoforms.[provided by RefSeq, May 2011]
Function Receptor involved in the costimulatory signal essential for T-lymphocyte proliferation and interleukin-2 production, by binding CD28 or CTLA-4. May play a critical role in the early events of T-cell activation and costimulation of naive T-cells, such as deciding between immunity and anergy that is made by T-cells within 24 hours after activation. Isoform 2 interferes with the formation of CD86 clusters, and thus acts as a negative regulator of T-cell activation. [UniProt]
Calculated MW 38 kDa
PTM Polyubiquitinated; which is promoted by MARCH8 and results in endocytosis and lysosomal degradation. [UniProt]

Images (3) Click the Picture to Zoom In

  • ARG23462 anti-CD86 antibody [24F] IHC-Fr image

    Immunohistochemistry: Rat lymph node cryosection stained with ARG23462 anti-CD86 antibody [24F] followed by HRP-conjugated Goat anti Mouse IgG. (Low power).

  • ARG23462 anti-CD86 antibody [24F] IHC-Fr image

    Immunohistochemistry: Rat lymph node cryosection stained with ARG23462 anti-CD86 antibody [24F] followed by HRP-conjugated Goat anti Mouse IgG. (Medium power).

  • ARG23462 anti-CD86 antibody [24F] IHC-Fr image

    Immunohistochemistry: Rat lymph node cryosection stained with ARG23462 anti-CD86 antibody [24F] followed by HRP-conjugated Goat anti Mouse IgG. (High power).