ARG42412

anti-CD85g / ILT7 antibody [17G10.2]

anti-CD85g / ILT7 antibody [17G10.2] for Flow cytometry and Human

Overview

Product Description Mouse Monoclonal antibody [17G10.2] recognizes CD85g / ILT7
Tested Reactivity Hu
Tested Application FACS
Specificity The mouse monoclonal antibody 17G10.2 recognizes an extracellular epitope of CD85g / ILT7, a member of leukocyte immunoglobulin-like receptor family expressed on plasmacytoid dendritic cells, but not on myeloid dendritic cells and other peripheral blood leukocytes.
Host Mouse
Clonality Monoclonal
Clone 17G10.2
Isotype IgG1, kappa
Target Name CD85g / ILT7
Antigen Species Human
Immunogen Human CD85g / ILT7–FcεRIγ-transfected 2B4 cells.
Conjugation Un-conjugated
Alternate Names ILT7; ILT-7; CD85 antigen-like family member G; Immunoglobulin-like transcript 7; CD antigen CD85g; CD85g; Leukocyte immunoglobulin-like receptor subfamily A member 4

Application Instructions

Application Suggestion
Tested Application Dilution
FACS2 - 5 µg/ml
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purification with Protein A.
Buffer PBS and 15 mM Sodium azide.
Preservative 15 mM Sodium azide
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 23547 Human LILRA4

Swiss-port # P59901 Human Leukocyte immunoglobulin-like receptor subfamily A member 4

Gene Symbol LILRA4
Gene Full Name leukocyte immunoglobulin-like receptor, subfamily A (with TM domain), member 4
Background This gene encodes an immunoglobulin-like cell surface protein that is expressed predominantly on plasmacytoid dendritic cells (PDCs) and modulates the function of these cells in the immune response. Expression of this gene is downregulated by interleukin 3 (IL3). This gene is one of a cluster of highly related genes located at chromosomal region 19q13.4. [provided by RefSeq, Jan 2015]
Function Functions coreceptor to limit the innate immune responses to viral infections; signaling occurs via FCER1G (PubMed:16735691, PubMed:19564354). Down-regulates the production of IFNA1, IFNA2, IFNA4, IFNB1 and TNF by plasmacytoid dendritic cells that have been exposed to influenza virus or cytidine-phosphate-guanosine (CpG) dinucleotides, indicating it functions as negative regulator of TLR7 and TLR9 signaling cascades (PubMed:16735691, PubMed:19564354, PubMed:24586760). Down-regulates interferon production in response to interaction with BST2 on HIV-1 infected cells (PubMed:26172439). Activates a signaling cascade in complex with FCER1G that results in phosphorylation of Src family and Syk kinases and thereby triggers mobilization of intracellular Ca(2+) (PubMed:16735691, PubMed:19564354). Does not interfere with the differentiation of plasmacytoid dendritic cells into antigen-presenting cells (PubMed:24586760). [UniProt]
Cellular Localization Cell membrane; Single-pass type I membrane protein. [UniProt]
Calculated MW 55 kDa

Images (1) Click the Picture to Zoom In

  • ARG42412 anti-CD85g / ILT7 antibody [17G10.2] FACS image

    Flow Cytometry: Human buffy coat cells stained with ARG42412 anti-CD85g / ILT7 antibody [17G10.2], followed by APC-conjugated Goat anti-Mouse antibody. Samples were co-stained with anti-CD123 antibody (FITC).