ARG65623
anti-CD82 antibody [C33] (low endotoxin)
anti-CD82 antibody [C33] (low endotoxin) for Flow cytometry,Functional study,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation,Western blot and Human
Cancer antibody; Gene Regulation antibody; Immune System antibody
Overview
| Product Description | Low endotoxin Mouse Monoclonal antibody [C33] recognizes CD82 (low endotoxin) |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | FACS, FuncSt, ICC/IF, IHC-P, IP, WB |
| Specificity | This antibody reacts with CD82, a widely expressed cell surface protein of the tetraspanin family. CD82 is also found in endosome/lysosome compartments. |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | C33 |
| Isotype | IgG2a |
| Target Name | CD82 |
| Antigen Species | Human |
| Immunogen | C91/PL (human HTLV-1+ T cell line) |
| Conjugation | Un-conjugated |
| Alternate Names | Tetraspanin-27; C33 antigen; R2; Tspan-27; TSPAN27; KAI1; CD antigen CD82; CD82 antigen; ST6; 4F9; Suppressor of tumorigenicity 6 protein; SAR2; IA4; Metastasis suppressor Kangai-1; Inducible membrane protein R2; GR15; C33 |
Application Instructions
| Application Suggestion |
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| Application Note | Functional application: In human MOLT-4 T-cell line the antibody C33 inhibits syncytium formation induced by coculture with human T-cell leukemia virus type 1 (HTLV-1)-positive human T-cell lines. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein A. |
| Purification Note | 0.2 µm filter sterilized. Endotoxin level is <0.01 EU/µg of the protein. |
| Purity | > 95% (by SDS-PAGE) |
| Buffer | PBS (pH 7.4) |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | CD82 |
| Gene Full Name | CD82 molecule |
| Background | This metastasis suppressor gene product is a membrane glycoprotein that is a member of the transmembrane 4 superfamily. Expression of this gene has been shown to be downregulated in tumor progression of human cancers and can be activated by p53 through a consensus binding sequence in the promoter. Its expression and that of p53 are strongly correlated, and the loss of expression of these two proteins is associated with poor survival for prostate cancer patients. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
| Function | Associates with CD4 or CD8 and delivers costimulatory signals for the TCR/CD3 pathway. [UniProt] |
| Research Area | Cancer antibody; Gene Regulation antibody; Immune System antibody |
| Calculated MW | 30 kDa |
Images (3) Click the Picture to Zoom In
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ARG65623 anti-CD82 antibody [C33] (low endotoxin) IHC-P image
Immunohistochemistry: Paraffin-embedded Human tonsil tissue stained with ARG65623 anti-CD82 antibody [C33] (low endotoxin).
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ARG65623 anti-CD82 antibody [C33] (low endotoxin) FACS image
Flow Cytometry: Human peripheral blood stained with ARG65623 anti-CD82 antibody [C33] (low endotoxin) at 1 µg/ml dilution, followed by APC-conjugated Goat anti-Mouse antibody.
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ARG65623 anti-CD82 antibody [C33] (low endotoxin) FACS image
Flow Cytometry: Separation of human CD82 positive lymphocytes (red-filled) from CD82 negative lymphocytes (black-dashed). Human peripheral whole blood stained with ARG65623 anti-CD82 antibody [C33] (low endotoxin) at 1 µg/ml dilution, followed by APC-conjugated Goat anti-Mouse antibody.
