ARG41596

anti-CD82 antibody

anti-CD82 antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat

Overview

Product Description Rabbit Polyclonal antibody recognizes CD82
Tested Reactivity Hu, Ms, Rat
Tested Application ICC/IF, IHC-P, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name CD82
Antigen Species Human
Immunogen Recombinant fusion protein corresponding to aa. 111-228 of Human CD82 (NP_002222.1).
Conjugation Un-conjugated
Alternate Names Tetraspanin-27; C33 antigen; R2; Tspan-27; TSPAN27; KAI1; CD antigen CD82; CD82 antigen; ST6; 4F9; Suppressor of tumorigenicity 6 protein; SAR2; IA4; Metastasis suppressor Kangai-1; Inducible membrane protein R2; GR15; C33

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IF1:50 - 1:200
IHC-P1:50 - 1:200
WB1:500 - 1:2000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control Rat kidney
Observed Size ~ 39 kDa

Properties

Form Liquid
Purification Affinity purified.
Buffer PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol.
Preservative 0.02% Sodium azide
Stabilizer 50% Glycerol
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 12521 Mouse CD82

GeneID: 3732 Human CD82

Swiss-port # P27701 Human CD82 antigen

Swiss-port # P40237 Mouse CD82 antigen

Gene Symbol CD82
Gene Full Name CD82 molecule
Background This metastasis suppressor gene product is a membrane glycoprotein that is a member of the transmembrane 4 superfamily. Expression of this gene has been shown to be downregulated in tumor progression of human cancers and can be activated by p53 through a consensus binding sequence in the promoter. Its expression and that of p53 are strongly correlated, and the loss of expression of these two proteins is associated with poor survival for prostate cancer patients. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
Function Associates with CD4 or CD8 and delivers costimulatory signals for the TCR/CD3 pathway. [UniProt]
Cellular Localization Membrane; Multi-pass membrane protein. [UniProt]
Calculated MW 30 kDa

Images (2) Click the Picture to Zoom In

  • ARG41596 anti-CD82 antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Mouse brain tissue stained with ARG41596 anti-CD82 antibody at 1:100 dilution.

  • ARG41596 anti-CD82 antibody WB image

    Western blot: 25 µg of Rat kidney lysate stained with ARG41596 anti-CD82 antibody at 1:3000 dilution.