Overview

Product Description	Rabbit Polyclonal antibody recognizes CD81
Tested Reactivity	Hu, Ms
Tested Application	ICC/IF, IHC-P, WB
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Target Name	CD81
Antigen Species	Human
Immunogen	Recombinant protein corresponding to Human CD81.
Conjugation	Un-conjugated
Alternate Names	CD antigen CD81; TAPA1; Tspan-28; S5.7; CD81 antigen; Target of the antiproliferative antibody 1; Tetraspanin-28; 26 kDa cell surface protein TAPA-1; CVID6; TSPAN28

Application Instructions

Application Suggestion	Tested Application Dilution ICC/IF 1:50 - 1:200 IHC-P 1:50 - 1:100 WB 1:500 - 1:1000
Application Note	* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control	MCF7
Observed Size	26 kDa

Properties

Form	Liquid
Purification	Affinity purified.
Buffer	50 mM Tris-Glycine (pH 7.4), 150 mM NaCl, 0.01% Sodium azide, 40% Glycerol and 0.05% BSA.
Preservative	0.01% Sodium azide
Stabilizer	40% Glycerol and 0.05% BSA
Concentration	Batch dependent
Storage Instruction	For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note	For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links	GeneID: 12520 Mouse CD81 GeneID: 975 Human CD81 Swiss-port # P35762 Mouse CD81 antigen Swiss-port # P60033 Human CD81 antigen
Gene Symbol	CD81
Gene Full Name	CD81 molecule
Background	The protein encoded by this gene is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. This encoded protein is a cell surface glycoprotein that is known to complex with integrins. This protein appears to promote muscle cell fusion and support myotube maintenance. Also it may be involved in signal transduction. This gene is localized in the tumor-suppressor gene region and thus it is a candidate gene for malignancies. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2014]
Function	May play an important role in the regulation of lymphoma cell growth. Interacts with a 16-kDa Leu-13 protein to form a complex possibly involved in signal transduction. May act as the viral receptor for HCV. [UniProt]
Cellular Localization	Basolateral cell membrane; Multi-pass membrane protein. Note=Associates with CLDN1 and the CLDN1-CD81 complex localizes to the basolateral cell membrane. [UniProt]
Calculated MW	26 kDa
PTM	Not glycosylated. [UniProt]

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