ARG53905

anti-CD80 antibody [MEM-233] (PerCP)

anti-CD80 antibody [MEM-233] (PerCP) for Flow cytometry and Human

Developmental Biology antibody; Immune System antibody

Overview

Product Description PerCP-conjugated Mouse Monoclonal antibody [MEM-233] recognizes CD80
Tested Reactivity Hu
Tested Application FACS
Specificity The clone MEM-233 reacts with CD80 (B7-1), a 60 kDa single chain type I glycoprotein of immunoglobulin supergene family, expressed on professional antigen-presenting cells, such as dendritic cells, macrophages or activated B lymphocytes.
Host Mouse
Clonality Monoclonal
Clone MEM-233
Isotype IgG1
Target Name CD80
Antigen Species Human
Immunogen Extracellular domain of human CD80 fused to human IgG1(Fc)
Conjugation PerCP
Alternate Names B7.1; CTLA-4 counter-receptor B7.1; CD28LG; T-lymphocyte activation antigen CD80; B7-1; CD28LG1; B7; LAB7; Activation B7-1 antigen; CD antigen CD80; BB1

Application Instructions

Application Suggestion
Tested Application Dilution
FACS10 µl / 10^6 cells
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Note The purified antibody is conjugated with Peridinin-chlorophyll-protein complex (PerCP) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary.
Buffer PBS, 15 mM Sodium azide and 0.2% (w/v) high-grade protease free BSA
Preservative 15 mM Sodium azide
Stabilizer 0.2% (w/v) high-grade protease free BSA
Storage Instruction Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 941 Human CD80

Swiss-port # P33681 Human T-lymphocyte activation antigen CD80

Gene Symbol CD80
Gene Full Name CD80 molecule
Background CD80 (B7-1) and CD86 (B7-2) are ligands of T cell critical costimulatory molecule CD28 and of an inhibitory receptor CTLA-4 (CD152). The both B7 molecules are expressed on professional antigen-presenting cells and are essential for T cell activation, the both molecules can also substitute for each other in this process. The question what are the differences in CD80 and CD86 competency has not been fully elucidated yet; there are still conflicts in results about their respective roles in initiation or sustaining of the T cell immune response.
Function Involved in the costimulatory signal essential for T-lymphocyte activation. T-cell proliferation and cytokine production is induced by the binding of CD28, binding to CTLA-4 has opposite effects and inhibits T-cell activation. [UniProt]
Research Area Developmental Biology antibody; Immune System antibody
Calculated MW 33 kDa

Images (1) Click the Picture to Zoom In

  • ARG53905 anti-CD80 antibody [MEM-233] (PerCP) FACS image

    Flow Cytometry: Separation of unstained (black) and stained (red) CD80 transfected P815 cells. Cells were stained with ARG53905 anti-CD80 antibody [MEM-233] (PerCP) at 9 µg/ml dilution.

Clone References

Immunophenotypic heterogeneity of bone marrow-derived mesenchymal stromal cells from patients with hematologic disorders: correlation with bone marrow microenvironment.

Campioni D et al.
Haematologica.,  (2006)

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