ARG62932

anti-CD80 antibody [16-10A1]

anti-CD80 antibody [16-10A1] for CyTOF®-candidate,Flow cytometry,Functional study,ICC/IF,IHC-Frozen sections,Immunoprecipitation and Mouse,Dog

Developmental Biology antibody; Immune System antibody

Overview

Product Description Hamster Monoclonal antibody [16-10A1] recognizes CD80
Tested Reactivity Ms, Dog
Tested Application CyTOF®-candidate, FACS, FuncSt, ICC/IF, IHC-Fr, IP
Specificity The clone 16-10A1 reacts with CD80 (B7-1), a 60 kDa single chain type I glycoprotein of immunoglobulin supergene family, expressed on professional antigen-presenting cells, such as dendritic cells, macrophages or activated B lymphocytes.
Host Hamster
Clonality Monoclonal
Clone 16-10A1
Isotype IgG2
Target Name CD80
Antigen Species Mouse
Immunogen Mouse CD80-transfected CHO cell line.
Conjugation Un-conjugated
Alternate Names B7.1; CTLA-4 counter-receptor B7.1; CD28LG; T-lymphocyte activation antigen CD80; B7-1; CD28LG1; B7; LAB7; Activation B7-1 antigen; CD antigen CD80; BB1

Application Instructions

Application Suggestion
Tested Application Dilution
CyTOF®-candidateAssay-dependent
FACS1 - 4 µg/ml
FuncStAssay-dependent
ICC/IFAssay-dependent
IHC-FrAssay-dependent
IPAssay-dependent
Application Note Functional study: Functions as blocking.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purified from hybridoma culture supernatant by protein-A affinity chromatography.
Purity > 95% (by SDS-PAGE)
Buffer PBS (pH 7.4) and 15 mM Sodium azide
Preservative 15 mM Sodium azide
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 12519 Mouse CD80

Swiss-port # Q00609 Mouse T-lymphocyte activation antigen CD80

Gene Symbol Cd80
Gene Full Name CD80 antigen
Background CD80 (B7-1) and CD86 (B7-2) are ligands of T cell critical costimulatory molecule CD28 and of an inhibitory receptor CTLA-4 (CD152). The both B7 molecules are expressed on professional antigen-presenting cells and are essential for T cell activation, the both molecules can also substitute for each other in this process. The question what are the differences in CD80 and CD86 competency has not been fully elucidated yet; there are still conflicts in results about their respective roles in initiation or sustaining of the T cell immune response.
Function Involved in the costimulatory signal essential for T lymphocytes activation. T-cell proliferation and cytokine production is induced by the binding of CD28 or CTLA-4 to this receptor. [UniProt]
Highlight Related products:
CD80 antibodies; CD80 ELISA Kits; Anti-Hamster IgG secondary antibodies;
Related news:
CyTOF-candidate Antibodies
Research Area Developmental Biology antibody; Immune System antibody
Calculated MW 33 kDa

Images (2) Click the Picture to Zoom In

  • ARG62932 anti-CD80 antibody [16-10A1] FACS image

    Flow Cytometry: Murine peripheral whole blood stained with ARG62932 anti-CD80 antibody [16-10A1] at 2 µg/ml dilution, followed by APC-conjugated Goat anti-Mouse antibody.

  • ARG62932 anti-CD80 antibody [16-10A1] FACS image

    Flow Cytometry: Separation of murine CD80 positive monocytes (red-filled) from CD80 negative lymphocytes (black-dashed). Murine peritoneal fluid cells stained with ARG62932 anti-CD80 antibody [16-10A1] at 2 µg/ml dilution, followed by APC-conjugated Goat anti-Mouse antibody.

Clone References

NF-kappaB2 is required for the control of autoimmunity by regulating the development of medullary thymic epithelial cells.

Zhang B et al.
J Biol Chem.,  (2006)

publication_link

 

hr_line

Modulation of cell cycle progression by CTLA4-CD80/CD86 interactions on CD4+ T cells depends on strength of the CD3 signal: critical role for IL-2.

Mukherjee S et al.
J Leukoc Biol.,  (2006)

publication_link

 

hr_line

Glomerular expression of CD80 and CD86 is required for leukocyte accumulation and injury in crescentic glomerulonephritis.

Odobasic D et al.
J Am Soc Nephrol.,  (2005)

publication_link

 

hr_line

Induction of B7-1 in podocytes is associated with nephrotic syndrome.

Reiser J et al.
J Clin Invest.,  (2004)

publication_link

 

hr_line

Bordetella bronchiseptica persists in the nasal cavities of mice and triggers early delivery of dendritic cells in the lymph nodes draining the lower and upper respiratory tract.

Gueirard P et al.
Infect Immun.,  (2003)

publication_link

 

hr_line