ARG22257
anti-CD74 antibody [PIN.1]
anti-CD74 antibody [PIN.1] for Flow cytometry,ICC/IF,Immunohistochemistry,Immunoprecipitation,Western blot and Human,Mouse
Overview
| Product Description | Mouse Monoclonal antibody [PIN.1] recognizes CD74 |
|---|---|
| Tested Reactivity | Hu, Ms |
| Tested Application | FACS, ICC/IF, IHC, IP, WB |
| Specificity | Detects ~33-35kDa protein doublet corresponding to the molecular mass of the p33 and p35 forms of human CD74. |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | PIN.1 |
| Isotype | IgG |
| Target Name | CD74 |
| Antigen Species | Human |
| Immunogen | Human CD74 invariant chain synthetic peptide |
| Conjugation | Un-conjugated |
| Alternate Names | DHLAG; Ii; CD antigen CD74; II; HLA-DR antigens-associated invariant chain; Ia-GAMMA; Ia antigen-associated invariant chain; HLA class II histocompatibility antigen gamma chain; p33; HLADG |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein G. |
| Buffer | PBS (pH 7.2), 0.09% Sodium azide and 50% Glycerol |
| Preservative | 0.09% Sodium azide |
| Stabilizer | 50% Glycerol |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P04233 Human HLA class II histocompatibility antigen gamma chain Swiss-port # P04441 Mouse H-2 class II histocompatibility antigen gamma chain |
|---|---|
| Gene Symbol | CD74 |
| Gene Full Name | CD74 molecule, major histocompatibility complex, class II invariant chain |
| Background | The protein encoded by this gene associates with class II major histocompatibility complex (MHC) and is an important chaperone that regulates antigen presentation for immune response. It also serves as cell surface receptor for the cytokine macrophage migration inhibitory factor (MIF) which, when bound to the encoded protein, initiates survival pathways and cell proliferation. This protein also interacts with amyloid precursor protein (APP) and suppresses the production of amyloid beta (Abeta). Multiple alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Aug 2011] |
| Function | Plays a critical role in MHC class II antigen processing by stabilizing peptide-free class II alpha/beta heterodimers in a complex soon after their synthesis and directing transport of the complex from the endoplasmic reticulum to the endosomal/lysosomal system where the antigen processing and binding of antigenic peptides to MHC class II takes place. Serves as cell surface receptor for the cytokine MIF. [UniProt] |
| Cellular Localization | Cell membrane, Endoplasmic Reticulum, Endoplasmic reticulum membrane, Endosome, Golgi apparatus, Lysosome |
| Calculated MW | 34 kDa |
| PTM | N- and O-glycosylated. O-glycosylated with core 1 or possibly core 8 glycans. |
Images (1) Click the Picture to Zoom In
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ARG22257 anti-CD74 antibody [PIN.1] ICC/IF image
Immunocytochemistry: 2% Formaldehyde (20 min at RT) fixed HeLa cells stained with ARG22257 anti-CD74 antibody [PIN.1] (green) at 1:100 dilution (12 hours at 4°C). Counterstain: DAPI (blue) nuclear stain at 1:40000 for 120 min at RT. Magnification: 100x. Left: DAPI (blue) nuclear stain, Middle: Primary antibody, Right: Composite.
