ARG22178
anti-CD74 antibody [LN2] (low endotoxin)
anti-CD74 antibody [LN2] (low endotoxin) for Blocking,Flow cytometry,ICC/IF,IHC-Frozen sections,IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation,Western blot and Human
Overview
| Product Description | Azide free and low endotoxin Mouse Monoclonal antibody [LN2] recognizes CD74 |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | BL, FACS, ICC/IF, IHC-Fr, IHC-P, IP, WB |
| Specificity | Human CD74 |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | LN2 |
| Isotype | IgG1, kappa |
| Target Name | CD74 |
| Antigen Species | Human |
| Immunogen | Cell line SU-DHL-4 nuclei |
| Conjugation | Un-conjugated |
| Alternate Names | DHLAG; Ii; CD antigen CD74; II; HLA-DR antigens-associated invariant chain; Ia-GAMMA; Ia antigen-associated invariant chain; HLA class II histocompatibility antigen gamma chain; p33; HLADG |
Application Instructions
| Application Suggestion |
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| Application Note | Blocking: The clone LN2 blocks MIF inducing B-lymphoma cell line positive proliferation. (PMID: 12782713) * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification Note | Low endotoxin |
| Buffer | PBS |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P04233 Human HLA class II histocompatibility antigen gamma chain |
|---|---|
| Gene Symbol | CD74 |
| Gene Full Name | CD74 molecule, major histocompatibility complex, class II invariant chain |
| Background | The protein encoded by this gene associates with class II major histocompatibility complex (MHC) and is an important chaperone that regulates antigen presentation for immune response. It also serves as cell surface receptor for the cytokine macrophage migration inhibitory factor (MIF) which, when bound to the encoded protein, initiates survival pathways and cell proliferation. This protein also interacts with amyloid precursor protein (APP) and suppresses the production of amyloid beta (Abeta). Multiple alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Aug 2011] |
| Function | Plays a critical role in MHC class II antigen processing by stabilizing peptide-free class II alpha/beta heterodimers in a complex soon after their synthesis and directing transport of the complex from the endoplasmic reticulum to the endosomal/lysosomal system where the antigen processing and binding of antigenic peptides to MHC class II takes place. Serves as cell surface receptor for the cytokine MIF. [UniProt] |
| Calculated MW | 34 kDa |
| PTM | N- and O-glycosylated. O-glycosylated with core 1 or possibly core 8 glycans. |
