ARG55075
anti-CD73 antibody
anti-CD73 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
Cell Biology and Cellular Response antibody
Overview
| Product Description | Mouse Monoclonal antibody recognizes CD73 |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | IHC-P, WB |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | 974CT7.5.3 |
| Isotype | IgG1 |
| Target Name | CD73 |
| Antigen Species | Human |
| Immunogen | Purified His-tagged Human CD73 protein. |
| Conjugation | Un-conjugated |
| Alternate Names | 5'-nucleotidase; CD antigen CD73; eN; CALJA; CD73; EC 3.1.3.5; NT; Ecto-5'-nucleotidase; NT5; E5NT; 5'-NT; eNT; NTE |
Application Instructions
| Application Suggestion |
|
||||||
|---|---|---|---|---|---|---|---|
| Application Note | IHC-P: Antigen Retrieval: Heat mediated was performed in Citrate buffer (pH 6.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
||||||
| Positive Control | WiDr |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein G. |
| Buffer | Crude Ascites and 0.09% (W/V) Sodium azide |
| Preservative | 0.09% (W/V) Sodium azide |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | NT5E |
| Gene Full Name | 5'-nucleotidase, ecto (CD73) |
| Background | The protein encoded by this gene is a plasma membrane protein that catalyzes the conversion of extracellular nucleotides to membrane-permeable nucleosides. The encoded protein is used as a determinant of lymphocyte differentiation. Defects in this gene can lead to the calcification of joints and arteries. Two transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Mar 2011] |
| Function | Hydrolyzes extracellular nucleotides into membrane permeable nucleosides. Exhibits AMP-, NAD-, and NMN-nucleosidase activities. [UniProt] |
| Cellular Localization | Cell membrane; Lipid-anchor, GPI-anchor. |
| Research Area | Cell Biology and Cellular Response antibody |
| Calculated MW | 63 kDa |
Images (2) Click the Picture to Zoom In
-
ARG55075 anti-CD73 antibody IHC-P image
Immunohistochemistry: Paraformaldehyde-fixed and paraffin-embedded Human kidney blocked with 3% BSA for 30 min at RT. Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0). Samples were stained with ARG55075 anti-CD73 antibody at 1:25 for 1 hours at 37°C.
-
ARG55075 anti-CD73 antibody WB image
Western blot: 20 µg of WiDr whole cell lysate stained with ARG55075 anti-CD73 antibody at 1:2000 dilution.
