ARG54839

anti-CD73 antibody

anti-CD73 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse

Cell Biology and Cellular Response antibody

Overview

Product Description

Rabbit Polyclonal antibody recognizes CD73

Tested Reactivity Hu, Ms
Tested Application IHC-P, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name CD73
Antigen Species Human
Immunogen KLH-conjugated synthetic peptide corresponding to aa. 520-550 (C-terminus) of Human CD73.
Conjugation Un-conjugated
Alternate Names 5'-nucleotidase; CD antigen CD73; eN; CALJA; CD73; EC 3.1.3.5; NT; Ecto-5'-nucleotidase; NT5; E5NT; 5'-NT; eNT; NTE

Application Instructions

Application Suggestion
Tested Application Dilution
IHC-PAssay-dependent
WB1:1000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control HepG2

Properties

Form Liquid
Purification Purification with Protein G.
Buffer PBS and 0.09% (W/V) Sodium azide
Preservative 0.09% (W/V) Sodium azide
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 23959 Mouse NT5E

GeneID: 4907 Human NT5E

Swiss-port # P21589 Human 5'-nucleotidase

Swiss-port # Q61503 Mouse 5'-nucleotidase

Gene Symbol NT5E
Gene Full Name 5'-nucleotidase, ecto (CD73)
Background The protein encoded by this gene is a plasma membrane protein that catalyzes the conversion of extracellular nucleotides to membrane-permeable nucleosides. The encoded protein is used as a determinant of lymphocyte differentiation. Defects in this gene can lead to the calcification of joints and arteries. Two transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Mar 2011]
Function Hydrolyzes extracellular nucleotides into membrane permeable nucleosides. Exhibits AMP-, NAD-, and NMN-nucleosidase activities. [UniProt]
Cellular Localization Cell membrane; Lipid-anchor, GPI-anchor.
Research Area Cell Biology and Cellular Response antibody
Calculated MW 63 kDa

Images (2) Click the Picture to Zoom In

  • ARG54839 anti-CD73 antibody IHC-P image

    Immunohistochemistry: Formalin-fixed and paraffin-embedded Human placenta tissue stained with ARG54839 anti-CD73 antibody.

  • ARG54839 anti-CD73 antibody WB image

    Western blot: 20 µg of HepG2 cell lysate stained with ARG54839 anti-CD73 antibody at 1:500 dilution.