ARG55404

anti-CD73 antibody [AD2]

anti-CD73 antibody [AD2] for CyTOF®-candidate,Flow cytometry,IHC-Frozen sections and Human,Primates

Cell Biology and Cellular Response antibody

Overview

Product Description Mouse Monoclonal antibody [AD2] recognizes CD73
Tested Reactivity Hu, NHuPrm
Tested Application CyTOF®-candidate, FACS, IHC-Fr
Specificity The mouse monoclonal antibody AD2 recognizes CD73, a 70 kDa GPI-anchored 5'-nucleotidase expressed predominantly on T and B cell subsets, follicular dendritic cells and endothelial cells.
Host Mouse
Clonality Monoclonal
Clone AD2
Isotype IgG1, kappa
Target Name CD73
Antigen Species Human
Immunogen Pre-B leukemia cells.
Conjugation Un-conjugated
Alternate Names 5'-nucleotidase; CD antigen CD73; eN; CALJA; CD73; EC 3.1.3.5; NT; Ecto-5'-nucleotidase; NT5; E5NT; 5'-NT; eNT; NTE

Application Instructions

Application Suggestion
Tested Application Dilution
CyTOF®-candidateAssay-dependent
FACS1 - 4 µg/ml
IHC-FrAssay-dependent
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Purification Purification with Protein A.
Purity > 95% (by SDS-PAGE)
Buffer PBS (pH 7.4) and 15 mM Sodium azide
Preservative 15 mM Sodium azide
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 4907 Human NT5E

Swiss-port # P21589 Human 5'-nucleotidase

Gene Symbol NT5E
Gene Full Name 5'-nucleotidase, ecto (CD73)
Background The protein encoded by this gene is a plasma membrane protein that catalyzes the conversion of extracellular nucleotides to membrane-permeable nucleosides. The encoded protein is used as a determinant of lymphocyte differentiation. Defects in this gene can lead to the calcification of joints and arteries. Two transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Mar 2011]
Function Hydrolyzes extracellular nucleotides into membrane permeable nucleosides. Exhibits AMP-, NAD-, and NMN-nucleosidase activities. [UniProt]
Highlight Related products:
CD73 antibodies; CD73 ELISA Kits; Anti-Mouse IgG secondary antibodies;
Related news:
CyTOF-candidate Antibodies
Research Area Cell Biology and Cellular Response antibody
Calculated MW 63 kDa

Images (1) Click the Picture to Zoom In

  • ARG55404 anti-CD73 antibody [AD2] CyTOF image

    CyTOF: PBMC (after Ficoll-Paque separation) stained with ARG55404 anti-CD73 antibody [AD2] (Yb176).