ARG42312
anti-CD73 antibody [AD2] (APC)
anti-CD73 antibody [AD2] (APC) for Flow cytometry and Human
Overview
| Product Description | APC-conjugated Mouse Monoclonal antibody [AD2] recognizes CD73 |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | FACS |
| Specificity | The mouse monoclonal antibody AD2 recognizes CD73, a 70 kDa GPI-anchored 5´-nucleotidase expressed predominantly on the surface of T and B cell subsets, follicular dendritic cells and endothelial cells. |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | AD2 |
| Isotype | IgG1, kappa |
| Target Name | CD73 |
| Antigen Species | Human |
| Immunogen | Human CD73. |
| Conjugation | APC |
| Alternate Names | 5'-nucleotidase; CD antigen CD73; eN; CALJA; CD73; EC 3.1.3.5; NT; Ecto-5'-nucleotidase; NT5; E5NT; 5'-NT; eNT; NTE |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified |
| Buffer | PBS and 15 mM Sodium azide. |
| Preservative | 15 mM Sodium azide |
| Storage Instruction | Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | NT5E |
| Gene Full Name | 5'-nucleotidase, ecto (CD73) |
| Background | The protein encoded by this gene is a plasma membrane protein that catalyzes the conversion of extracellular nucleotides to membrane-permeable nucleosides. The encoded protein is used as a determinant of lymphocyte differentiation. Defects in this gene can lead to the calcification of joints and arteries. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2011] |
| Function | Hydrolyzes extracellular nucleotides into membrane permeable nucleosides. Exhibits AMP-, NAD-, and NMN-nucleosidase activities. [UniProt] |
| Cellular Localization | Cell membrane; Lipid-anchor, GPI-anchor. [UniProt] |
| Calculated MW | 63 kDa |
Images (3) Click the Picture to Zoom In
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ARG42312 anti-CD73 antibody [AD2] (APC) FACS image
Flow Cytometry: Human peripheral whole blood stained with ARG42312 anti-CD73 antibody [AD2] (APC) at 10 µl / 100 µl of peripheral whole blood.
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ARG42312 anti-CD73 antibody [AD2] (APC) FACS image
Flow Cytometry: Human lymphocytes stained with ARG42312 anti-CD73 antibody [AD2] (APC) at 10 µl / 100 µl of peripheral whole blood and ARG53783 anti-CD19 antibody [LT19] (PE) at 20 µl / 100 µl of peripheral whole blood.
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ARG42312 anti-CD73 antibody [AD2] (APC) FACS image
Flow Cytometry: Separation of Human CD73 positive CD19 positive B cells (red-filled) from neutrophil granulocytes (black-dashed). Human peripheral whole blood stained with ARG42312 anti-CD73 antibody [AD2] (APC) at 10 µl / 100 µl of peripheral whole blood.
