ARG10510
anti-CD71 / Transferrin Receptor (soluble) antibody [13E4]
anti-CD71 / Transferrin Receptor (soluble) antibody [13E4] for ELISA,Western blot and Human
Cancer antibody; Cell Biology and Cellular Response antibody; Developmental Biology antibody; Immune System antibody; Metabolism antibody
Overview
| Product Description | Mouse Monoclonal antibody [13E4] recognizes CD71 / Transferrin Receptor (soluble) |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA, WB |
| Specificity | Human soluble transferrin receptor and complex between soluble transferrin receptor and transferrin |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | 13E4 |
| Isotype | IgG2a |
| Target Name | CD71 / Transferrin Receptor (soluble) |
| Antigen Species | Human |
| Immunogen | Balb/c mice immunized with purified human soluble transferrin receptor. |
| Conjugation | Un-conjugated |
| Alternate Names | TFR1; CD antigen CD71; CD71; T9; p90; TR; Trfr; Transferrin receptor protein 1; TRFR; sTfR; TfR1; TfR; TFR |
Application Instructions
| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
|---|
Properties
| Form | Liquid |
|---|---|
| Purification | Protein A affinity purified. |
| Buffer | PBS (pH 7.2) and 0.1% Sodium azide |
| Preservative | 0.1 % Sodium azide |
| Concentration | 1.0-2.0 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | TFRC |
| Gene Full Name | transferrin receptor |
| Function | Cellular uptake of iron occurs via receptor-mediated endocytosis of ligand-occupied transferrin receptor into specialized endosomes. Endosomal acidification leads to iron release. The apotransferrin-receptor complex is then recycled to the cell surface with a return to neutral pH and the concomitant loss of affinity of apotransferrin for its receptor. Transferrin receptor is necessary for development of erythrocytes and the nervous system (By similarity). A second ligand, the heditary hemochromatosis protein HFE, competes for binding with transferrin for an overlapping C-terminal binding site. [UniProt] |
| Highlight | Related Antibody Duos and Panels: ARG30200 Transferrin Receptor ELISA Antibody Duo Related products: CD71 antibodies; CD71 ELISA Kits; CD71 Duos / Panels; Anti-Mouse IgG secondary antibodies; |
| Research Area | Cancer antibody; Cell Biology and Cellular Response antibody; Developmental Biology antibody; Immune System antibody; Metabolism antibody |
| Calculated MW | 85 kDa |
| PTM | N- and O-glycosylated, phosphorylated and palmitoylated. The serum form is only glycosylated. Proteolytically cleaved on Arg-100 to produce the soluble serum form (sTfR). Palmitoylated on both Cys-62 and Cys-67. Cys-62 seems to be the major site of palmitoylation. |
Clone References
A human blood-brain barrier transcytosis assay reveals antibody transcytosis influenced by pH-dependent receptor binding.
TBC1D14 regulates autophagosome formation via Rab11- and ULK1-positive recycling endosomes.
