ARG23215

anti-CD71 / Transferrin Receptor antibody [YTA74.4]

anti-CD71 / Transferrin Receptor antibody [YTA74.4] for Flow cytometry,IHC-Frozen sections,Immunoprecipitation and Mouse

Overview

Product Description Rat Monoclonal antibody [YTA74.4] recognizes CD71 / Transferrin Receptor
Rat anti Mouse CD71 antibody, clone YTA74. 4 recognizes the mouse Transferrin receptor protein 1 also known as CD71 or TfR1. CD71 is a 763 amino acid glycoprotein homodimer of ~95 kDa subunits. CD71 is expressed by dividing cells, and functions as a transferrin receptor mediating uptake of iron. Rat anti Mouse CD71 antibody, clone YTA74. 4 blocks the binding of R17 217. 1. 3. and R17 208. 2 anti-TFR monoclonal antibodies (Trowbridge et al. 1982).
Tested Reactivity Ms
Tested Application FACS, IHC-Fr, IP
Host Rat
Clonality Monoclonal
Clone YTA74.4
Isotype IgG2a
Target Name CD71 / Transferrin Receptor
Antigen Species Mouse
Immunogen Concanavilin A activated Mouse spleen cells.
Conjugation Un-conjugated
Alternate Names TFR1; CD antigen CD71; CD71; T9; p90; TR; Trfr; Transferrin receptor protein 1; TRFR; sTfR; TfR1; TfR; TFR

Application Instructions

Application Suggestion
Tested Application Dilution
FACS1:50 - 1:100
IHC-FrAssay-dependent
IPAssay-dependent
Application Note FACS: Use 10 µl of the suggested working dilution to label 10^6 cells in 100 µl.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purification with Protein G.
Buffer PBS and 0.09% Sodium azide.
Preservative 0.09% Sodium azide
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 22042 Mouse TFRC

Swiss-port # Q62351 Mouse Transferrin receptor protein 1

Gene Symbol TFRC
Gene Full Name transferrin receptor
Background This gene encodes a cell surface receptor necessary for cellular iron uptake by the process of receptor-mediated endocytosis. This receptor is required for erythropoiesis and neurologic development. Multiple alternatively spliced variants have been identified. [provided by RefSeq, Sep 2015]
Function Cellular uptake of iron occurs via receptor-mediated endocytosis of ligand-occupied transferrin receptor into specialized endosomes. Endosomal acidification leads to iron release. The apotransferrin-receptor complex is then recycled to the cell surface with a return to neutral pH and the concomitant loss of affinity of apotransferrin for its receptor. Transferrin receptor is necessary for development of erythrocytes and the nervous system (By similarity). A second ligand, the heditary hemochromatosis protein HFE, competes for binding with transferrin for an overlapping C-terminal binding site. [UniProt]
Calculated MW 85 kDa
PTM N- and O-glycosylated, phosphorylated and palmitoylated. The serum form is only glycosylated.

Proteolytically cleaved on Arg-100 to produce the soluble serum form (sTfR).

Palmitoylated on both Cys-62 and Cys-67. Cys-62 seems to be the major site of palmitoylation. [UniProt]