ARG41865
anti-CD71 / Transferrin Receptor antibody
anti-CD71 / Transferrin Receptor antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
Overview
| Product Description | Rabbit Polyclonal antibody recognizes CD71 / Transferrin Receptor |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | ICC/IF, IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | CD71 / Transferrin Receptor |
| Antigen Species | Human |
| Immunogen | Recombinant fusion protein corresponding to aa. 1-140 of Human CD71 / Transferrin Receptor. (NP_001121620.1) |
| Conjugation | Un-conjugated |
| Alternate Names | TFR1; CD antigen CD71; CD71; T9; p90; TR; Trfr; Transferrin receptor protein 1; TRFR; sTfR; TfR1; TfR; TFR |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||
| Positive Control | Mouse liver | ||||||||
| Observed Size | ~ 100 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purified. |
| Buffer | PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 50% Glycerol |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | TFRC |
| Gene Full Name | transferrin receptor |
| Background | This gene encodes a cell surface receptor necessary for cellular iron uptake by the process of receptor-mediated endocytosis. This receptor is required for erythropoiesis and neurologic development. Multiple alternatively spliced variants have been identified. [provided by RefSeq, Sep 2015] |
| Function | Cellular uptake of iron occurs via receptor-mediated endocytosis of ligand-occupied transferrin receptor into specialized endosomes. Endosomal acidification leads to iron release. The apotransferrin-receptor complex is then recycled to the cell surface with a return to neutral pH and the concomitant loss of affinity of apotransferrin for its receptor. Transferrin receptor is necessary for development of erythrocytes and the nervous system (By similarity). A second ligand, the heditary hemochromatosis protein HFE, competes for binding with transferrin for an overlapping C-terminal binding site. [UniProt] |
| Cellular Localization | Cell membrane; Single-pass type II membrane protein. Melanosome. Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV. Transferrin receptor protein 1, serum form: Secreted. [UniProt] |
| Calculated MW | 85 kDa |
| PTM | N- and O-glycosylated, phosphorylated and palmitoylated. The serum form is only glycosylated. Proteolytically cleaved on Arg-100 to produce the soluble serum form (sTfR). Palmitoylated on both Cys-62 and Cys-67. Cys-62 seems to be the major site of palmitoylation. [UniProt] |
Images (4) Click the Picture to Zoom In
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ARG41865 anti-CD71 / Transferrin Receptor antibody ICC/IF image
Immunofluorescence: L929 cells stained with ARG41865 anti-CD71 / Transferrin Receptor antibody at 1:100 dilution.
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ARG41865 anti-CD71 / Transferrin Receptor antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Rat kidney tissue stained with ARG41865 anti-CD71 / Transferrin Receptor antibody 1:100 dilution.
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ARG41865 anti-CD71 / Transferrin Receptor antibody WB image
Western blot: 25 µg of Mouse liver lysate stained with ARG41865 anti-CD71 / Transferrin Receptor antibody at 1:3000 dilution.
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ARG41865 anti-CD71 / Transferrin Receptor antibody IP image
Immunoprecipitation: 200 µg extracts of Jurkat cells were immunoprecipitated and stained with ARG41865 anti-CD71 / Transferrin Receptor antibody at 1:1000 dilution.
