ARG65360
anti-CD64 antibody [10.1] (low endotoxin)
anti-CD64 antibody [10.1] (low endotoxin) for Flow cytometry,Functional study,ICC/IF,IHC-Frozen sections,IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation,Western blot and Human,Primates
Immune System antibody
Overview
| Product Description | Azide free and low endotoxin Mouse Monoclonal antibody [10.1] recognizes CD64 |
|---|---|
| Tested Reactivity | Hu, NHuPrm |
| Tested Application | FACS, FuncSt, ICC/IF, IHC-Fr, IHC-P, IP, WB |
| Specificity | The clone 10.1 recognizes alpha subunit of CD64/FcgammaRI, a 72 kDa single chain type I glycoprotein, that is expressed on monocytes/macrophages, dendritic cells, and activated granulocytes. HLDA III; WS Code M-250 |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | 10.1 |
| Isotype | IgG1 |
| Target Name | CD64 |
| Antigen Species | Human |
| Immunogen | Rheumatoid synovial fluid cells and fibronectin purified human monocytes |
| Conjugation | Un-conjugated |
| Alternate Names | High affinity immunoglobulin gamma Fc receptor I; CD64; Fc-gamma RIA; CD antigen CD64; FcgammaRIa; FCRI; IgG Fc receptor I; CD64A; Fc-gamma RI; FcRI; IGFR1 |
Application Instructions
| Application Suggestion |
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| Application Note | IHC-P and IHC-Fr: There can occur problems with paraformaldehyde fixation. Functional studies: Blocking of IgG binding to the FcgammaR1. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein A. |
| Purification Note | 0.2 µm filter sterilized. Endotoxin level is <0.01 EU/µg of the protein, as determined by the LAL test. |
| Purity | > 95% (by SDS-PAGE) |
| Buffer | PBS (pH 7.4) |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P12314 Human High affinity immunoglobulin gamma Fc receptor I |
|---|---|
| Gene Symbol | FCGR1A |
| Gene Full Name | Fc fragment of IgG, high affinity Ia, receptor (CD64) |
| Background | CD64 (FcgammaRI) is a cell surface receptor for Fc region of IgG. It is composed of specific ligand binding alpha subunit and promiscuous gamma subunit, which is indispensable for tyrosine-based signaling. However, even the alpha subunit can transduce signals leading to cellular effector functions. The isoform FcgammaRIa1 binds human IgG with high affinity, has limited myeloid cell distribution, and a relatively large intracellular domain. Products of related genes include FcgammaRIb and FcgammaRIc isoforms, but these specify low affinity IgG receptors if functionally expressed at all. Besides a role in antigen clearance, FcgammaRI (a1) can potently enhance MHC class I and II antigen presentation in vitro and in vivo. |
| Function | High affinity receptor for the Fc region of immunoglobulins gamma. Functions in both innate and adaptive immune responses. [UniProt] |
| Research Area | Immune System antibody |
| Calculated MW | 43 kDa |
| PTM | Phosphorylated on serine residues. |
Images (2) Click the Picture to Zoom In
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ARG65360 anti-CD64 antibody [10.1] (low endotoxin) FACS image
Flow Cytometry: Human peripheral blood stained with ARG65360 anti-CD64 antibody [10.1] (low endotoxin) at 4 µg/ml dilution, followed by APC-conjugated Goat anti-Mouse antibody.
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ARG65360 anti-CD64 antibody [10.1] (low endotoxin) FACS image
Flow Cytometry: Separation of human monocytes (red-filled) from CD64 negative lymphocytes (black-dashed). Human peripheral whole blood stained with ARG65360 anti-CD64 antibody [10.1] (low endotoxin) at 4 µg/ml dilution, followed by APC-conjugated Goat anti-Mouse antibody.
Clone References
Rap1 activation is required for Fc gamma receptor-dependent phagocytosis.
FACS /
CD16+ and CD16- human blood monocyte subsets differentiate in vitro to dendritic cells with different abilities to stimulate CD4+ T cells.
Intracellular cytokine profile of cord and adult blood monocytes.
Upgrading of flow cytometric analysis for absolute counts, cytokines and other antigenic molecules of cynomolgus monkeys (Macaca fascicularis) by using anti-human cross-reactive antibodies.
High affinity IgG binding by FcgammaRI (CD64) is modulated by two distinct IgSF domains and the transmembrane domain of the receptor.
